The Life Imaging Center (LIC) is a central core facility of the Albert-Ludwigs-University Freiburg open to members of all Faculties and the Universitätsklinikum. Currently more than 280 active users from six Faculties (Biology, Medicine, Chemistry and Pharmacy, Environment and Natural Resources, Mathematics and Physics, and Engineering) use the LIC infrastructure. LIC users come from more than 90 workgroups and the combined usage time of the LIC microscopes in 2017 was about 10.350 hours. The LIC has approximately 60 - 80 new users per year and approximately the same number leaving the facility each year. Users from external institutions (e.g. Max Planck Institutes, Fraunhofer Institutes) and from national and international universities (e.g. Basel, Strasbourg, Haute-Alsace) are able to use the LIC infrastructure. More details about the application procedure and facility guidelines can be found at: https://miap.eu/miap-units/life-imaging-center-lic/application-to-lic/. You can find contact information for the head and staff of the LIC facility at: https://miap.eu/miap-units/life-imaging-center-lic/team/.
The Life Imaging Center (LIC) consists of 14 rooms in the ZBSA with approximately 400 m² (9 microscope rooms with 19 microscope set-ups, wet lab, large cell culture room, and large computer lab with 12 high-end workstations) plus 2 additional rooms with 3 LSMs (35 m²) in a neighboring building.
Techniques and Samples:
Most modern microscopic techniques (FRET, FRAP, FLIP, FLAP, photo-conversion, photo-activation, uncaging, spectral unmixing, multi-position and large area recording, TIRF, structured illumination, microinjection while imaging) can be performed in combination with live cells or organisms on the available microscope set-ups.
The LIC has a major focus on live cell imaging of all kind of cells, organisms and culture systems used in cell signaling research, developmental biology and neurobiology. Samples are: Embryos (Danio rerio, Drosophila melanogaster, Mus musculus, Xenopus laevis), model organisms or cell clusters (C.elegans, spheres, cysts), plants (root, leave, growth cone), neuronal cultures, explants and biopsies from tissue slices, spheroids, flow cultures as well as cells cultured on glass slides.
LIC is specialized in long time observation and experiments on these objects, high resolution large area and/or multiple location recordings of thick objects with 2P and UV/VIS excitation. A further specialty is culturing and permanent microscopic observation of cells and spheroids over periods of up to 10 days (also under flow conditions in special chambers).
All types of fluorescent proteins including many photo-activatable and photo-convertible are used. We have a large library of fluorescent proteins (more than 150) available for our users via the BIOSS toolbox and we perform test experiments with these constructs before giving them out to our customers. See also: Available excitation wavelengths for imaging at LIC (PDF).