Joseph Huff1*, Ingo Kleppe1, Angela Naumann2,3 and Roland Nitschke2,3 Nature Methods, 2018
Airyscan detection in multiphoton microscopy: super-resolution and improved signal-to-noise ratio beyond the confocal limit
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The penetration depth of traditional confocal laser-scanning microscopy (LSM) systems is limited by light scattering. To avoid these limitations, multiphoton LSM uses a nonlinear fluorophore excitation process in combination with a non-descanned detection concept to greatly increase the penetration depth. However, in traditional multiphoton LSM, this increased depth necessitates a compromise on the achievable spatial resolution and signal-to-noise compared with that of confocal LSM. The novel Airyscan detection concept from ZEISS, used in combination with multiphoton excitation, overcomes these limitations and provides increased resolution and signal-to-noise with a 2–3× increase in penetration depth compared with that of traditional confocal LSM.
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