Literature

Home / Resources / Literature

Airyscan detection in multiphoton microscopy: super-resolution and improved signal-to-noise ratio beyond the confocal limit

Joseph Huff1*, Ingo Kleppe1, Angela Naumann2,3 and Roland Nitschke2,3  Nature Methods, 2018
https://www.nature.com/articles/d42473-018-00102-3
Download PDF version

The penetration depth of traditional confocal laser-scanning microscopy (LSM) systems is limited by light scattering. To avoid these limitations, multiphoton LSM uses a nonlinear fluorophore excitation process in combination with a non-descanned detection concept to greatly increase the penetration depth. However, in traditional multiphoton LSM, this increased depth necessitates a compromise on the achievable spatial resolution and signal-to-noise compared with that of confocal LSM. The novel Airyscan detection concept from ZEISS, used in combination with multiphoton excitation, overcomes these limitations and provides increased resolution and signal-to-noise with a 2–3× increase in penetration depth compared with that of traditional confocal LSM.

 

Tridimensional Visualization and Analysis of Early Human Development

Belle et al, 2017
https://www.ncbi.nlm.nih.gov/pubmed/28340341

The authors provide an initial look at dynamic processes during early human development through 3D cellular imaging using light sheet microscopy.