Airyscan detection in multiphoton microscopy: super-resolution and improved signal-to-noise ratio beyond the confocal limit
Joseph Huff1*, Ingo Kleppe1, Angela Naumann2,3 and Roland Nitschke2,3 Nature Methods, 2018
Download PDF version
The penetration depth of traditional confocal laser-scanning microscopy (LSM) systems is limited by light scattering. To avoid these limitations, multiphoton LSM uses a nonlinear fluorophore excitation process in combination with a non-descanned detection concept to greatly increase the penetration depth. However, in traditional multiphoton LSM, this increased depth necessitates a compromise on the achievable spatial resolution and signal-to-noise compared with that of confocal LSM. The novel Airyscan detection concept from ZEISS, used in combination with multiphoton excitation, overcomes these limitations and provides increased resolution and signal-to-noise with a 2–3× increase in penetration depth compared with that of traditional confocal LSM.
Tridimensional Visualization and Analysis of Early Human Development
Belle et al, 2017
The authors provide an initial look at dynamic processes during early human development through 3D cellular imaging using light sheet microscopy.
Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb
Wolff et al, 2018, eLIFE
The authors imaged fluorescently-labeled Parhyale hawaiensis embryos using multi-view light-sheet microscopy to study the cellular basis of limb development.