Video Lectures and Tutorials

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Short Microscope Series from ibiology

This video lectures where produced by www.ibology.org.

The lectures give a good overview of light microscopy. The following topics are addressed:

basic of optics,
transmitted light,
fluorescence and GFP,
cofocal microscopy,
digital imaging and
rescent advances in super-resolution microscopy.

Microscope Series

14 Videos

Microscopy: What Can You Learn With a Light Microscope (Ron Vale)

19:22

Microscopy: Early History of Microscopy (Joseph Gall)

25:53

Microscopy: Resolution in Microscopy (Jeff Lichtman)

39:01

Microscopy: Dark Field, Phase Contrast, Polarization and DIC (Edward Salmon)

31:29

Microscopy: Lenses and Image Formation (Daniel Fletcher)

36:47

Microscopy: Microscope Imaging and Koehler Illumination (Ron Vale)

22:45

Microscopy: Cameras and Digital Image Analysis (Nico Stuurman)

33:41

Microscopy: Introduction to Fluorescence Microscopy (Nico Stuurman)

33:35

Microscopy: Fluorescent Probes (Timothy Mitchison)

20:47

Microscopy: Optical Sectioning and Confocal Microscopy (Kurt Thorn)

24:47

Microscopy: Measuring Dynamics: Photobleaching and Photoactivation (JLS)

26:30

Microscopy: Super-Resolution Microscopy (Xiaowei Zhuang)

37:36

Microscopy: Fluorescent Proteins (Roger Tsien)

34:37

Microscopy: Choosing the Right Microscopy Technique (Ron Vale)

29:08

QUAREP-LiMi

QUAREP-LiMi is a group of enthusiastic light microscopists from academia and industry all interested in improving quality assessment (QA) and quality control (QC) in light microscopy. There is also a YouTube channel.

QUAREP-LiMi Tutorials

4 Videos

Illumination Stability Measurement on a Spinning Disk Microscope

6:46

How to set up a Powermeter for illumination intensity measurement with a microscope objective

2:15

Laser Power readings on a Nikon confocal with a Thorlabs Power Meter

4:17

Laser Power stability measurement with a Phyton macro and ZEISS Zen Blue software

1:52

Optical Interest group

Janelia’s Optical Interest Group (OIG) connects scientists at Janelia with a common interest in optical imaging tools (microscope and labeling technology) and image analysis. The website can be found under:

https://www.janelia.org/content/optical-interest-group

There is also a YouTube channel.

Optical Interest Group Seminar Series

16 Videos

Virginie Uhlmann: "Quantifying Biological Shape Across Imaging Modalities"

56:50

Stephan Preibisch: “Reconstructing large-scale lightsheet microscopy acquisitions”

53:16

Jonas Ries: "Superresolution microscopy for structural cell biology"

55:57

Alison Tebo: “Imaging cellular biochemistry using chemical-genetic tools”

54:38

Lina Streich: "High-resolution deep brain imaging using adaptive optics three-photon microscopy"

55:55

Stephan Saalfeld: "Scalable open-source methods and tools for large microscopy volumes"

1:08:57

Claire Deo: "Engineering hybrid molecular reporters for biological imaging"

41:04

Helen Farrants and Yan Zhang, Janelia Research Campus

52:47

Anna Kreshuk: "Whole-organism segmentation: finding all cells in an EM-imaged platynereis dumerilii”

45:02

Kaspar Podgorski: "How programmable microscopes can improve activity imaging"

50:42

Jerome Mutterer and Wei Ouyan: "Why use ImageJ in 2021?"

1:29:22

Timo Zimmermann: "Introducing the EMBL Imaging Centre"

52:47

Isabel Espinosa Medina: "Temporal encoding and manipulation of vertebrate cell histories ..."

53:18

Adrian Wolny: “Embedding-based Instance Segmentation with Limited Supervision.”

1:03:39

Nicholas Sofroniew: "napari: a Python multi-dimensional image visualization and analysis..."

57:29

Carsen Stringer "Cellpose & Suite2p: anatomical and functional algorithms for cellular segmentation"

56:07

ZEISS Webinars and Tutorials

ZEISS offer a lot of tutorials and ZEN blue software training.

Down below two videos show you how to Koehler different type of ZEISS microscopes.

How to Koehler

5 Videos

Upright

5:01

Inverted

5:01

ZEISS Microscopy How-to: Set up Köhler Illumination on your ZEISS Axio Observer

5:01

Microscopy: Choosing the Right Microscopy Technique (Ron Vale)

29:08

ZEISS Microscopy How-to: Align and Focus your HAL Light Source for Köhler Illumination

2:43

Argolight Tutorials

Argolight offers a lot of tutorials for its calibration tools and Daybook software.

Down below you can watch the tutorials.

Argolight Tutorials

10 Videos

[How To] #1 Starting Procedure - Check your fluorescence Microscope with Argolight

1:40

[How To] #2 Illumination inhomogeneity - Check your fluorescence Microscope with Argolight

1:43

[How To] #3 Cleaning procedure - Check your fluorescence Microscope with Argolight

1:24

[Daybook 2] Part #1 Analyis

6:03

[Daybook 2] Part #2 Data Manager

4:16

[FAQ] What's the flatness of the Argolight slides ? - Argolight

0:58

[FAQ] Are there fluorophores inside your glass? - Argolight

0:44

[FAQ] Does fluorescence stay stable forever? - Argolight

0:50

[FAQ] Does fluorescence stay stable forever? - Argolight

0:51

[FAQ] What's the excitation wavelength? - Argolight

0:36

Omero tutorials

Omero offers a lot of tutorials how to the software.

Down below you can watch the tutorials.

Omero Tutorials

4 Videos

Import Data using OMERO.insight

8:33

OMERO.figure: Getting started

6:32

OMERO.figure comparing images

5:34

OMERO.figure scalebars and labels

4:43

Microscopy and Image Analysis Platform (MIAP)

A joint Network for scientific Imaging and Image Analysis Infrastructure

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