Imaging 4

Pos.	Objective	Manufacturer No.	Mag.	N.A.	WD mm	Immersion	DIC	Int. No.
1	EC Plan-Neofluar 5x/0.16 Ph1 M27	420331-9911	5	0.16	18.5		–	137
2	Plan-Apochromat 10x/0.45 M27	420640-9900-000	10	0.45	2		x II	172
3	LD Plan-Neofluar 20x/0.4 Korr Ph 2 M 27	421351-9970-000	20	0.4	7.9			224
4	Plan-Apochromat 63x/1.40 Oil DIC	420782-9900	63	1.4	0.19	Oil	III	170
5	α Plan-Apochromat 100x/1.46 Oil DIC (UV) VIS-IR	420792-9800	100	1.46	0.11	Oil	III	185
A	Fluar 100x/1.30 Oil UV	440285	100	1.3	0.17	Oil	x III	74
A	EC Plan-Neofluar 20x/0.5	420350-9900	20	0.5	2		–	155
A	Plan-Apochromat 20x/0.75	440649 (1101-957)	20	0.75	0.61		x II	3 ICF
A	EC Plan-Neofluar 10x/0.30 M27	420340-9901	10	0.3	5.2		–	167
A	LD LCI Plan-Apochromat 25x/0.80 Imm Korr DIC	420852-9870	25	0.8	0.57	H2O, Glyc, Oil	II	168
A	Fluar 40x/1.30 Oil DIC	420260-9900	40	1.3	0.16	Oil	III	169
A	Plan-Apochromat 5x/0.16	420630-9900	5	0.16	12.1			197
A/6	TIRF 1x/- calibration objektiv	423684-9000	1					171

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Pos.	Name	Excitation	DC/BS	Emission	Dye	Int. No.
1	FSet 49	BP 365	BS 395	BP 445/50	DAPI	068
2	FSet 46 HE	BP 500/25	BS 515	BP 535/30	GFP, YFP	069
3	FSet 43 HE	BP 550/25	BS 570	BP 605/70	RFP	070
5	DIC				DIC	072
6	FSet 64 HE	BP 587/25	BS 605	BP 647/70	mCherry	256
Box 09	FSet 48	BP 436/20	BS 455	BP 535/30	Ex. CFP, Em. YFP	011
Box 09	FSet 79 HE ms (1)		DBS 490+575		GFP+RFP Laser	077
Box 09	CFP	BP 436/10	BS 460	BP 480/50	CFP	124
Box 09	pHluorin Chroma custom		BS T495lpxr	BP 535/50	pHluorin	246
Box 10	FSet 78 HE ms	BP 445/25, BP 510/15	DBS 460 + 520		CFP+YFP dual camera	074
Box 10	FSet 78 HE (2)		BS 510	BP 480/22 + LP 530	CFP+YFP dual camera	076
Box 10	NT		50/50			078
Box 10	TIRF				CFP+YFP	079
Box 10	FSet 47 HE	BP 436/25	BS 455	BP 480/40	CFP	117
Box 11	FSet 38 HE	BP 470/40	BS 495	BP 525/50	GFP	071
Box 11	TIRF 488	BP 488/10 ZET	BS 488 ZT488rdc-UF2	BP 525/50m ET + LP 500 ET	GFP TIRF	275
Box 11	TIRF 561	BP 554/23	BS 561 ZT561rdc-UF2	BP 600/50m ET + LP 575 ET	RFP	276
Dual Kamera Port	FSet 79 HE ms	DBP 470/27 + 556/25	DBS 490 + 575, BS 565	BP 630/98	GFP+RFP	080
stock	F37-488	BP 488/6			BCECF	113
stock	FSet 78 ms	BP 445/25			CFP	114
stock	F27-436	BP 436/10			BCECF	116
stock	FSet 79 ms (1)	BP 470/27			GFP	118
stock	FSet 79 ms (1)	BP 556/25			RFP	119
stock	BP 340/30 for FSet 21 HE	BP 340/30			Fura-2	120
stock	BP 387/15 for FSet 21 HE	BP 387/15			Fura-2	121
stock	BP 510/15 for FSet 78 ms	BP 510/15			YFP	122
stock	FF01-406/15	BP 406/15			pHluorin	244
stock	pHluorin Chroma custom	BP 473/10 (Semrock)			pHluorin	248
	TIRF+Weitfeld Dual	DBP 482/563	BS 405-488/568rpc-UF2	DBP 523/610	GFP+RFP TIRF	277
	FSet 92 HE LED		TBS 405 + 493 + 610	TBS425/30 + 524/50 + 688/145		340

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Model name	Manufacturer	type	BW / Color	FPS	pix no. X	pix no. Y	pix size X (µm)	QE (%)
AxioCam MRm r3.0	Zeiss	CCD	BW	11	1388	1040	6.45	65
AxioCam MRm r3.0	Zeiss	CCD	BW	11	1388	1040	6.45	65
Evolve	Photometrics	emCCD	BW	33.7	512	512	16	95

Model name	Type	Spectral range (nm)
Colibri 7	LED	385, 430, 475, 511, 555, 590, 630
LGK 7877 ML01	Laser	458, 488, 514, 561

ZEISS AxioObserver with TIRF