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ZEISS Axio Observer with TIRF

Equipment type: Light microscopy

live cell time-lapse imaging set-up for multi-location 3D-XFP ratio imaging with

TIRF laser illumination (458, 488, 514 and 561 nm)
Sutter DG-4 wavelength switcher
Definite Focus II, motorized xy-table for multi location and tile-image recording
2 cooled CCD cameras (Axiocam Rev.3, 1388×1040)
EMCCD camera Evolve (optimized field of view 512×512 imaging array)
Software ZenBlue (Zeiss)

(FRISYS funding to M. Simons collaboration with R.N.)

Location

Room 00.018
Phone: +49 761 203-97123
Life Imaging Center
Germany

Contact Specialist

Life Imaging Center team
Phone: +49 761 203-2934
Email: lic@imaging.uni-freiburg.de

Usage Fee

12 € / hour
Special discounts might be applicable.
We may charge you for additional components.
For details check our usage fee overview or
the usage conditions document.

Documentation

Objektive_Filter_Imaging 4_TIRF
Turn on tutorial Imaging 1
Picture Story Imaging 4

ZEISS Axio Observer Information

Objectives

Pos.	Objective	Manufacturer No.	Mag.	N.A.	WD mm	Immersion	DIC	Int. No.
2	C-Achroplan 40x/0.80 W NIR	1080-378	40	0.8	3.61	H2O	x III	122
3	LD LCI Plan-Apochromat 40x/1.20 autocorr	420862-9970-790	40	1.2	0.41	H2O, Glyc	x III	251
4	LD LCI Plan-Apochromat 25x/0.80 Imm Korr (UV)VIS-IR	420852-9870	25	0.8	0.57	H2O, Glyc, Oil	x II	217
5	Plan-Apochromat 10x/0.45	440639	10	0.45	2.8		–	66
6	Plan-Apochromat 63x/1.40 Oil DIC M27	420782-9900	63	1.4	0.19	Oil	x III	121
A	Plan-Apochromat 20x/0.80	420650-9901-000	20	0.8	0.55		x II	236
A	C-Achroplan 32x/0.85 W Korr M27 VIS-IR	420967-9970-000	32	0.85	1.1	H2O		237
A	LD C-Apochromat 63x/1.14 W Korr UV-VIS-IR	421887-9970-000	63	1.14	0.6	H2O	x III	239
A	EC Epiplan Neofluar 5x/0.13 DIC M27	000000-1156-511	5	0.13	15.8		–	240

Filters

Pos.	Name	Excitation	DC/BS	Emission	Dye	Int. No.
1	FSet 49	BP 365	BS 395	BP 445/50	DAPI	068
2	FSet 46 HE	BP 500/25	BS 515	BP 535/30	GFP, YFP	069
3	FSet 43 HE	BP 550/25	BS 570	BP 605/70	RFP	070
5	DIC				DIC	072
6	FSet 64 HE	BP 587/25	BS 605	BP 647/70	mCherry	256
Box 09	FSet 48	BP 436/20	BS 455	BP 535/30	Ex. CFP, Em. YFP	011
Box 09	FSet 79 HE ms (1)		DBS 490+575		GFP+RFP Laser	077
Box 09	CFP	BP 436/10	BS 460	BP 480/50	CFP	124
Box 09	pHluorin Chroma custom		BS T495lpxr	BP 535/50	pHluorin	246
Box 10	FSet 78 HE ms	BP 445/25, BP 510/15	DBS 460 + 520		CFP+YFP dual camera	074
Box 10	FSet 78 HE (2)		BS 510	BP 480/22 + LP 530	CFP+YFP dual camera	076
Box 10	NT		50/50			078
Box 10	TIRF				CFP+YFP	079
Box 10	FSet 47 HE	BP 436/25	BS 455	BP 480/40	CFP	117
Box 11	FSet 38 HE	BP 470/40	BS 495	BP 525/50	GFP	071
Box 11	TIRF 488	BP 488/10 ZET	BS 488 ZT488rdc-UF2	BP 525/50m ET + LP 500 ET	GFP TIRF	275
Box 11	TIRF 561	BP 554/23	BS 561 ZT561rdc-UF2	BP 600/50m ET + LP 575 ET	RFP	276
Dual Kamera Port	FSet 79 HE ms	DBP 470/27 + 556/25	DBS 490 + 575, BS 565	BP 630/98	GFP+RFP	080
stock	F37-488	BP 488/6			BCECF	113
stock	FSet 78 ms	BP 445/25			CFP	114
stock	F27-436	BP 436/10			BCECF	116
stock	FSet 79 ms (1)	BP 470/27			GFP	118
stock	FSet 79 ms (1)	BP 556/25			RFP	119
stock	BP 340/30 for FSet 21 HE	BP 340/30			Fura-2	120
stock	BP 387/15 for FSet 21 HE	BP 387/15			Fura-2	121
stock	BP 510/15 for FSet 78 ms	BP 510/15			YFP	122
stock	FF01-406/15	BP 406/15			pHluorin	244
stock	pHluorin Chroma custom	BP 473/10 (Semrock)			pHluorin	248
	TIRF+Weitfeld Dual	DBP 482/563	BS 405-488/568rpc-UF2	DBP 523/610	GFP+RFP TIRF	277
	FSet 92 HE LED		TBS 405 + 493 + 610	TBS425/30 + 524/50 + 688/145		340

Cameras

Model name	Manufacturer	type	BW / Color	FPS	pix no. X	pix no. Y	pix size X (µm)	QE (%)
AxioCam MRm r3.0	Zeiss	CCD	BW	11	1388	1040	6.45	65
AxioCam MRm r3.0	Zeiss	CCD	BW	11	1388	1040	6.45	65
Evolve	Photometrics	emCCD	BW	33.7	512	512	16	95

Light Sources

Model name	Type	Spectral range (nm)
Colibri 7	LED	385, 430, 475, 511, 555, 590, 630
LGK 7877 ML01	Laser	458, 488, 514

Microscopy and Image Analysis Platform (MIAP)

A joint Network for scientific Imaging and Image Analysis Infrastructure

Get in touch:

Phone: +49 761 203 2934
Email: info@miap.eu
Contact Form

How to find us:

Albert-Ludwigs Universität Freiburg
Signalling Campus Freiburg (SCF)
Habsburgerstr. 49
79104 Freiburg im Breisgau
Germany

Imaging 4

Home / Imaging 4

ZEISS Axio Observer with TIRF

Equipment type: Light microscopy

Location

Contact Specialist

Usage Fee

Documentation

Microscopy and Image Analysis Platform (MIAP)

Get in touch:

How to find us:

© 2020 MIAP, University of Freiburg

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Imaging 4

Home / Imaging 4

ZEISS Axio Observer with TIRF

Equipment type: Light microscopy

Location

Contact Specialist

Usage Fee

Documentation

Microscopy and Image Analysis Platform (MIAP)

Get in touch:

How to find us:

© 2020 MIAP, University of Freiburg

ImpressumPrivacy PolicyCookie Policy

Impressum
Privacy Policy
Cookie Policy