Objectives
| Position | Objective | Objective No. | Mag. | N.A. | WD mm | Imm. | DIC | Int. No. |
|---|---|---|---|---|---|---|---|---|
| 1 | EC Plan-Neofluar 5x/0,16 Ph1 M27 | 420331-9911 | 5 | 0.16 | 18.5 | – | – | 137 LIC |
| 2 | Plan-Apochromat 10x/0,45 M27 | 420640-9900-000 | 10 | 0.45 | 2 | – | x | 172 WD |
| 3 | LD Plan-Neofluar 20x/0,4 Korr Ph 2 M 27 | 421351-9970-000 | 20 | 0.4 | 7.9 | – | – | 224 F |
| 4 | Plan Apochromat 63x/1,40 Oil DIC | 420782-9900 | 63 | 1.4 | 0.19 | Oil | III | 170 FRI |
| 5 | α Plan-Apochromat 100x/1,46 oil DIC (UV) VIS-IR | 420792-9800 | 100 | 1.46 | 0.11 | Oil | III | 185 LIC |
| A | Fluar 100x/1,30 Oil UV | 440285 | 100 | 1.3 | 0.17 | Oil | x III | 74 Ni SFB |
| A | EC Plan-Neofluar 20x/0,5 | 420350-9900 | 20 | 0.5 | 2 | – | 155 Ni | |
| A | Plan Apochromat 20x/0,75 | 440649 (1101-957) | 20 | 0.75 | 0.61 | – | xII | 3ICF |
| A | EC Plan Neofluar 10x/0,30 M27 | 420340-9901 | 10 | 0.3 | 5.2 | – | – | 167 FRI |
| A | LD LCI Plan-Apochromat 25x/0,80 DIC Imm Korr | 420852-9870 | 25 | 0.8 | 0.57 | H2O, Oil, Glyc | II | 168 FRI |
| A | Fluar 40x/1,30 Oil DIC | 420260-9900 | 40 | 1.3 | 0.16 | Oil | III | 169 FRI |
| A | Plan-Apochromat 5x/0,16 | 420630-9900 | 5 | 0.16 | 12.1 | – | – | 197 Ni |
| A/6 | TIRF 1x/- calibration objektiv | 423684-9000 | 1 | – | – | 171 FRI |
Filters
| Position | Name | Excitation | DC/BS | Emission | Dye | Filter-No. | Link |
|---|---|---|---|---|---|---|---|
| Dual Kamera Port | FSet 79 HE ms | BP 470/27(HE), BP 556/25 (HE) | DBS 490 + 575 (HE), BS 565 (HE) | BP 630/98 (HE) | GFP+RFP | 080 | Zeiss FS 79 |
| Filterbox 09 | FSet 48 | BP 436/20 | BS 455 | BP 535/30 | Ex. CFP, Em. YFP | 011 | Zeiss FS 48 |
| Filterbox 09 | Cy5 HC | HC 628/40 | BS Q 660 LP | BP 700/75 HQ | Cy 5, Alexa 647 | 016 | |
| Filterbox 09 | FSet 79 HE ms (1) | DBS 490+575 | GFP+RFP Laser | 077 | |||
| Filterbox 09 | CFP | BP 436/10 | BS 460 | BP 480/50 | CFP | 124 | |
| Filterbox 09 | pHluorin Chroma custom | BS 495lpxr T | BP 535/50 ET | pHluorin | 246 | ||
| Filterbox 10 | FSet 78 HE ms | BP 445/25, BP 510/15 | DBS 460 + 520, BS 510 | BP 480/22, LP 530 | CFP+YFP dual camera | 074 | Zeiss FS 78 HE ms |
| Filterbox 10 | FSet 78 HE (2) | BP 480/22 | BS 510 | LP 530 | CFP+YFP dual camera | 076 | |
| Filterbox 10 | Mirror | Mirror 50/50 | 078 | ||||
| Filterbox 10 | TIRF | CFP+YFP | 079 | ||||
| Filterbox 10 | FSet 47 HE | BP 436/25 | BS 455 | BP 480/40 | CFP | 117 | Zeiss FS 47 HE |
| Filterbox 10 | TIRF+Weitfeld Dual | DBP 482/563 | DC 405-488/568rpc-UF2 | DBP 523/610 F57-488 | GFP+RFP TIRF | 277 | Semrock FF01-554-23 |
| Filterbox 11 | FSet 38 HE | BP 470/40 | BS 495 | BP 525/50 | GFP | 071 | Zeiss FS 38 HE |
| Filterbox 11 | TIRF 488 | BP 488/10 ZET | DC 488 ZT488rdc-uF2 | ET 525/50m + LP 500 ET | GFP TIRF | 275 | Semrock FF01-482/563 |
| Filterbox 11 | TIRF 561 | BP 554/23 | DC 561 ZT561rdc-UF2 | ET 600/50m + LP 575 ET | RFP | 276 | Semrock FF01-523/610 |
| Pos. 1 | FSet 49 | BP 365 | BS 395 | BP 445/50 | Dapi | 068 | Zeiss FS 49 |
| Pos. 2 | FSet 46 HE | BP 500/25 | BS 515 | BP 535/30 | YFP | 069 | Zeiss FS 46 HE |
| Pos. 3 | FSet 43 HE | BP 550/25 | BS 570 | BP 605/70 | RFP | 070 | Zeiss FS 43 HE |
| Pos. 5 | DIC | DIC | 072 | ||||
| Pos. 6 | FSet 64 HE | BP 587/25 | BS 605 | BP 647/70 | mCherry | 256 | Zeiss FS 64 |
| Schrank | F37-488 | BP 488/6 | BCECF | 113 | AHF F37-488 | ||
| Schrank | FSet 78 ms | BP 445/25 | CFP | 114 | Zeiss FS 78 | ||
| Schrank | F27-436 | BP 436/10 | BCECF | 116 | AHF F27-436 similar | ||
| Schrank | FSet 79 ms 1 | BP 470/27 | GFP | 118 | Zeiss FS 79 | ||
| Schrank | FSet 79 ms 1 | BP 556/25 | RFP | 119 | Zeiss FS 79 | ||
| Schrank | BP 340/30 for FSet 21 HE | BP 340/30 | Fura-2 | 120 | Zeiss FS 21 HE | ||
| Schrank | BP 387/15 for FSet 21 HE | BP 387/15 | Fura-2 | 121 | Zeiss FS 21 HE | ||
| Schrank | BP 510/15 for FSet 78 ms | BP 510/15 | YFP | 122 | Zeiss FS 78 | ||
| Schrank | Semrock FF01-406/15 | BP 406/15 | pHluorin | 244 | |||
| Schrank | pHluorin Chroma custom | BP 473/10 (Semrock) | pHluorin | 248 |
Cameras
| Model name | manufactorer | type | bit depth (max) | pix no. X | pix no. Y | pix size X | pix size Y |
|---|---|---|---|---|---|---|---|
| AxioCam MRm | Zeiss | CCD | 12 | 1388 | 1040 | ||
| AxioCam MRm | Zeiss | CCD | 12 | 1388 | 1040 | ||
| Photometrics evolve | Photometrics | emCCD |
Exitation wavelengths
This microscope allows the following exication wavelengths:
- 458 nm
- 488 nm
- 514 nm
- 561 nm