Imaging 1

Pos.	Objective	Manufacturer No.	Mag.	N.A.	WD mm	Immersion	DIC	Int. No.
1	EC Plan-Neofluar 2.5x/0.085	420320-9902	2.5	0.085	8.8		–	231
2	Plan-Apochromat 20x/0.80 M27	420650-9901-000	20	0.8	0.55			223
3	Plan-Neofluar 5x/0.15	440320	5	0.15	13.6		–	4
4	Plan-Apochromat 10x/0.45	1063-139	10	0.45	2.8		x II	68
5	C-Apochromat 40x/1.20 W Korr	440052 (01)	40	1.2	0.29	H2O	x III	3
6	Plan-Apochromat 63x/1.40 Oil DIC M27	420782-9900-000	63	1.4	0.19	Oil	x II	226
A	Plan-Neofluar 10x/0.30	440330	10	0.3	5.6		–	5
A	Plan-Neofluar 100x/1.30 Oil	440480	100	1.3	0.2	Oil	x III	9
A	LD Achroplan 63x/0.75 Korr Ph 2	440861	63	0.75	1.57		–	24
A	C-Apochromat 63x/1.20 W Korr	440668 (01)	63	1.2	0.28	H2O	x III	44
A	LD A-Plan 40x/0.50 Ph 2	1006-595	40	0.5	2		x II	78
A	LD A-Plan 20x/0.30 Ph 1	1006-591	20	0.3	4.3		x II	79
A	LD LCI Plan-Apochromat 25x/0.80 DIC Imm Korr (UV) VIS-IR	440842-9870	25	0.8	0.57	H2O, Glyc, Oil	x II	161
A	A-Plan 10x/0.25 Ph1	441031-0000-000	10	0.25	4.5		–	222

Pos.	Name	Excitation	DC/BS	Emission	Dye	Int. No.
1	DIC				DIC	147
2	DAPI	BP 365	BS 410	BP 447/60-25	DAPI	160
3	FSet 61 HE (Colibri)	BP 474/28, BP 585/35	DBS 495 + 605	DBP 527/54+ 645/60	GFP+mCherry	263
4	GFP	BP 470/40 HQ	BS 500	BP 530/50	GFP	250
5	FSet 43 HE	BP 550/25	BS 570	BP 605/70	RFP	032
6	Cy 5	BP 640/30	BS 660	BP 690/50	Cy 5	253
Box 01	F36-508	BP 562/40	BS 593 HC	BP 641/75	mCherry	111
Box 01	Ex GFP/Em DsRED	BP 470/40	BS 562	LP 561	Ex. GFP, Em. RFP	139
Box 01	CFP	BP 406/15	BS 425 HC	BP 457/50 HC	CFP	149
Box 01	FSet 47 HE		BS 455	BP 480/40	CFP	150
Box 01	BS 425		BS 425 HC			151
Box 01	LP 515 ET		BS 515 ET LP	BP 535/30	GFP	152
Box 01	FSet 46 HE	BP 500/25	BS 515	BP 535/30	GFP, YFP	251

Model name	Manufacturer	type	BW / Color	FPS	pix no. X	pix no. Y	pix size X (µm)	QE (%)
AxioCam 105 color	Zeiss	CMOS	Color	15	2560	1920	2.2
AxioCam MRm r3.0	Zeiss	CCD	BW	11	1388	1040	6.45	65

ZEISS AxioObserver with Colibri 2