Conditions for LIC facility usage under the Corona pandemic
Dear users of the Life Imaging Center - LIC,
The facility is now fully open again and you can book all our systems 24/7 according to the LIC user agreement.
The following conditions apply:
A) The most important requirement for the continued successful operation of the facility is to keep the minimum distance (1.5 m), to wear a mouth-and-nose cover, lab coat and gloves when entering the LIC area as well as to obey strictly to the safety rules of SAR-CoV2 hygiene rules of the university and the Corona Verordnung of the state of Baden-Württemberg. For joint usage of instruments with two persons, contact the LIC staff.
B) Between the successive booking slots a break time of 30 minutes is still mandatory.
Your LIC and MIAP team.
Albert-Ludwigs University Freiburg
+49 761 203-2934
+49 761 203-2941
From Freiburg Hbf (Central station) take tram line 4 (to station: Hauptstraße), local train connections (direction Offenburg) to station Freiburg Herdern (only 1 station) or bus line 27 (to station: Herdern Kirche) and then by foot: 5 minutes
From south (e.g. Basel): Motorway A5 Basel-Karlsruhe; take gateway 62: „Freiburg Mitte” (Center). Take the B31a direction "Freiburg/Donaueschingen". Take B3 (exit "Merzhausen") and turn right onto Stefan-Meier Straße. After the prison turn right onto Tennenbacherstraße and then turn left on Habsburgerstraße.
From north (e.g. Karlsruhe): Motorway A5 Karlsruhe-Basel; take gateway 61: „Freiburg Nord” (North). Take the B294 direction "Freiburg-Nord/Waldkirch/Elztal/Glottertal/Gundelfingen". Follow B294/B3 until you arrive at Habsburgerstraße.
Approx. 1 hour bus ride from the EuroAirport Basel-Mulhouse-Freiburg or 2 to 3 hours by train or car from Frankfurt, Stuttgart or Zürich airports.
Take a Tour through the Life Imaging Center (LIC)
The Life Imaging Center, which is now one of the largest facilities of that type in Germany, was founded in 2000 by W. Driever and R. Nitschke as the central project Z2 of the SFB 592 "Signaling mechanisms in embryogenesis and organogenesis". The LIC was originally located in the Institute of Biology I and moved in April 2008 into the new building of the Zentrum für Biosystemanalyse (ZBSA) in Habsburgerstr. 49. With the move into the ZBSA building, the LIC also merged with the Imaging Core Facility of the Medical Faculty founded in 2003 by R. Nitschke and M. Frotscher. After a transition of the hosting ZBSA to the Center for Integrative Signalling Studies (CISA), the LIC got incorporated in the newly formed Signalling Campus Freiburg (SCF) in June 2020. The LIC works tightly together and shares equipment and software licenses with the Imaging facility of Max Planck Institute for Immunobiology and Epigenetics (MPI-IE) in Freiburg.
Life Imaging Center:
The Life Imaging Center (LIC) is a central core facility of the Albert-Ludwigs-University Freiburg open to members of all Faculties and the Universitätsklinikum. Currently more than 280 active users from six Faculties (Biology, Medicine, Chemistry and Pharmacy, Environment and Natural Resources, Mathematics and Physics, and Engineering) use the LIC infrastructure. LIC users come from more than 90 workgroups and the combined usage time of the LIC microscopes in 2017 was about 10.350 hours. The LIC has approximately 60 - 80 new users per year and approximately the same number leaving the facility each year. Users from external institutions (e.g. Max Planck Institutes, Fraunhofer Institutes) and from national and international universities (e.g. Basel, Strasbourg, Haute-Alsace) are able to use the LIC infrastructure. More details about the application procedure and facility guidelines can be found at: https://miap.eu/miap-units/freiburg/life-imaging-center-lic/application-to-lic. You can find contact information for the head and staff of the LIC facility at: https://miap.eu/miap-units/freiburg/life-imaging-center-lic/team.
The Life Imaging Center (LIC) consists of 14 rooms in the Center for Integrative Signalling Analysis (CISA) with approximately 400 m² (9 microscopy rooms with 20 microscope set-ups (3D-STED, confocal FLIM, two 2-photon confocal microscopes, 4 conventional confocal microscopes, lightsheet microscope, 4 high-end widefield ratio-imaging set-ups, 4 fluorescence stereo microscopes, 3 special set-ups for screening). The LIC microscopes are hosted in Biosafety level S2 and S1 environments, connected to a large wet lab area and a cell culture room, both Biosafety level S2 classified. Additionally, the LIC offers a large computer lab with 12 high-end workstations for image processing and analysis. The LIC also covers two additional rooms (35 m²), hosting 3 LSM systems in a neighboring building of the Signalling Campus Freiburg (SCF).
Techniques and Samples:
Most modern microscopic techniques (FRET, FRAP, FLIP, FLAP, photo-conversion, photo-activation, uncaging, spectral unmixing, multi-position and large area recording, TIRF, structured illumination, microinjection while imaging) can be performed in combination with live cells or organisms on the available microscope set-ups.
The LIC has a major focus on live cell imaging of all kind of cells, organisms and culture systems used in cell signaling research, developmental biology and neurobiology. Samples are: Embryos (Danio rerio, Drosophila melanogaster, Mus musculus, Xenopus laevis), model organisms or cell clusters (C.elegans, spheres, cysts), plants (root, leave, growth cone), neuronal cultures, explants and biopsies from tissue slices, spheroids, flow cultures as well as cells cultured on glass slides.
LIC is specialized in long time observation and experiments on these objects, high resolution large area and/or multiple location recordings of thick objects with 2P and UV/VIS excitation. A further specialty is culturing and permanent microscopic observation of cells and spheroids over periods of up to 10 days (also under flow conditions in special chambers).
All types of fluorescent proteins including many photo-activatable and photo-convertible are used. We have a large library of fluorescent proteins (more than 150) available for our users via the BIOSS toolbox and we perform test experiments with these constructs before giving them out to our customers. See also: Available excitation wavelengths for imaging at LIC (PDF).