Perkin Elmer Spinning Disk

Perkin Elmer spinning disk confocal "ULTRA VIEW VoX"

Equipment type: Light microscopy

SystemSpecification_PE
SYSTEM SPECIFICATIONS:
  • Imaging: Fast live imaging with low phototoxicity; FRAP
  • Stand: Leica DMI 6000B with adaptive focus control
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Photokinesis (FRAP) unit
  • Software: Volocity
CAMERA SPECIFICATIONS:
  • Model: two Hamamatsu C9100-50 frame transfer EMCCD cameras
  • Effective number of pixels: 1000 x 1000
  • Cell size: 8.0µm x 8.0µm
  • Effective Area: 8.0mm x 8.0mm
  • Max frame rate: 28.1 frames/second
  • Dynamic range: 14 bit
  • Quantum efficiency (peak): > 60%
  • Read out noise at min EM gain: 10 electrons
  • Read out noise at max EM gain: <1 electron
TWO ACQUISITION MODES AVAILABLE:
  1. SINGLE camera setup
  2. DUAL camera setup
OBJECTIVES
ObjectiveMagnification / NAMediumWorking Distance
HC PLAN APO10x / 0.40IMM0.36 mm
HC PLAN APO20x / 0.70IMM0.26 mm
HXC PLAN S APO40x / 1.25-0.75oil0.1 mm
HXC PLAN S APO63x / 1.40-0.60oil0.1 mm
U PLAN S APO100x / 1.40-0.70oil0.09 mm
HXC PLAN S APO63x / 1.20water0.22 mm
LASERLINES
FIXED wavelengthsAvailabilityLaser type and powerSome dyes and use
405nmYESsolid state laser, 50 mWDAPI
445nm*YESsolid state laser, 40 mWCyanFP
488nmYESsolid state laser, 50 mWGFP, Alexa488, CY2, OregonGreen
514nm*YESsolid state laser, 25 mWYellowFP
561nmYESsolid state laser, 50 mWRedFPs, Alexa568
640nmYESsolid state laser, 40 mWDRAQ5, CY5, Alexa648
*CFP/YFP imaging is available only on request.  
DICHROICS
DichroicAvailabilityExtra Emission Filter 1Extra Emission Filter 2Kit No
580 nm edgeYESdual band 594/42-730/140dual band 448/55-523/47L7268950
509 nm edgeOn demanddual band 594/42-730/140475/42L7268951
BANDPASS FILTERS (Emitters)
FluorochromesAvailabilityBandpassEmissionEm wheel No
DAPI / RFPYESDual-band445 / 60-615 / 70Set 2
CFP / Far-RedYESDual-band485 / 60-705 / 90Set 2
GFPYESSingle-band525 / 50Set 2
YFPYESSingle-band550 / 49Set 2

Location

Contact Specialist

Usage Fee

10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

No documents available