Perkin Elmer Spinning Disk

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Perkin Elmer spinning disk confocal "ULTRA VIEW VoX"

Equipment type: Light microscopy

SYSTEM SPECIFICATIONS:
  • Imaging: Fast live imaging with low phototoxicity; FRAP
  • Stand: Leica DMI 6000B with adaptive focus control
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Photokinesis (FRAP) unit
  • Software: Volocity
CAMERA SPECIFICATIONS:
  • Model: two Hamamatsu C9100-50 frame transfer EMCCD cameras
  • Effective number of pixels: 1000 x 1000
  • Cell size: 8.0µm x 8.0µm
  • Effective Area: 8.0mm x 8.0mm
  • Max frame rate: 28.1 frames/second
  • Dynamic range: 14 bit
  • Quantum efficiency (peak): > 60%
  • Read out noise at min EM gain: 10 electrons
  • Read out noise at max EM gain: <1 electron
TWO ACQUISITION MODES AVAILABLE:
  1. SINGLE camera setup
  2. DUAL camera setup
OBJECTIVES
Objective Magnification / NA Medium Working Distance
HC PLAN APO 10x / 0.40 IMM 0.36 mm
HC PLAN APO 20x / 0.70 IMM 0.26 mm
HXC PLAN S APO 40x / 1.25-0.75 oil 0.1 mm
HXC PLAN S APO 63x / 1.40-0.60 oil 0.1 mm
U PLAN S APO 100x / 1.40-0.70 oil 0.09 mm
HXC PLAN S APO 63x / 1.20 water 0.22 mm
LASERLINES
FIXED wavelengths Availability Laser type and power Some dyes and use
405nm YES solid state laser, 50 mW DAPI
445nm* YES solid state laser, 40 mW CyanFP
488nm YES solid state laser, 50 mW GFP, Alexa488, CY2, OregonGreen
514nm* YES solid state laser, 25 mW YellowFP
561nm YES solid state laser, 50 mW RedFPs, Alexa568
640nm YES solid state laser, 40 mW DRAQ5, CY5, Alexa648
*CFP/YFP imaging is available only on request.  
DICHROICS
Dichroic Availability Extra Emission Filter 1 Extra Emission Filter 2 Kit No
580 nm edge YES dual band 594/42-730/140 dual band 448/55-523/47 L7268950
509 nm edge On demand dual band 594/42-730/140 475/42 L7268951
BANDPASS FILTERS (Emitters)
Fluorochromes Availability Bandpass Emission Em wheel No
DAPI / RFP YES Dual-band 445 / 60-615 / 70 Set 2
CFP / Far-Red YES Dual-band 485 / 60-705 / 90 Set 2
GFP YES Single-band 525 / 50 Set 2
YFP YES Single-band 550 / 49 Set 2

Location

Contact Specialist

Usage Fee

10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

No documents available