3i Spinning Disk

Home / 3i Spinning Disk

3i spinning disk confocal "MARIANAS"

Equipment type: Light microscopy

SYSTEM SPECIFICATIONS:
  • Imaging: Fast live imaging with low phototoxicity; FRAP; FRET; Photoconversion
  • Stand: Zeiss Axio Observer
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: Slidebook 5
CAMERA SPECIFICATIONS:
  • Model: Photometrics Evolve 512, back-illuminated EMCCD
  • Effective number of pixels: 512 x 512
  • Cell size: 16.0µm x 16.0µm
  • Effective Area: 8.0mm x 8.0mm
  • Max frame rate: 33.7 frames/second
  • Dynamic range: 16 bit
  • Quantum efficiency (peak): > 90%
  • Read out noise at min EM gain: 5 electrons
  • Read out noise at max EM gain: <0.5 electron
OBJECTIVES
Objective Magnification / NA Medium Contrast Working Distance
EC Plan NeoFLUOR 40x / 0.75 water 0.7 mm
EC Plan NeoFLUOR 40x / 1.3 oil DIC 0.2 mm
PL APOCHROMAT 63x / 1.4 oil DIC 0.18 mm
PLAN APO 100x / 1.40 oil DIC 0.17 mm
LASERLINES
FIXED wavelengths Availability Laser type Some dyes and use
405nm YES diode laser DAPI
440 nm YES diode laser CyanFP
488nm YES diode laser GFP, Alexa488, CY2
514 nm YES diode laser YellowFP
555nm YES diode laser RedFPs, Alexa568
639nm YES diode laser DRAQ5, CY5, Alexa648
FILTERCUBES for Zeiss stand
Fluorochromes Availability Excitation Dichroic Emission Filter No
DAPI YES 387 / 11-25 409 447 / 60-25 DAPI-1160A
GFP YES 472 / 30-25 495 520 / 35-25 GFP-3035
CFP YES 438 / 24-25 458 483 / 32-25 CFP-2432
Cy3 YES 531 / 40-25 562 593 / 40-25 Cy3-4040A
Cy5 On Demand 628 / 40-25 660 692 / 40-25 Cy5-4040A
YFP On Demand 500 / 24-25 520 542 / 27-25 YFP-2427A
BANDPASS FILTERS (Emitters)
Fluorochromes Availability Bandpass Emission Position No
DAPI/FITC/Cy3/Cy5 YES Multi-band 440 / 521 / 607 / 700-25 1
DAPI On Demand Single-band 445 / 45-25 On Demand
CFP YES Single-band 482 / 35-25 3
GFP YES Single-band 525 / 30-25 4
YFP YES Single-band 542 / 27-25 5
RFP YES Single-band 617 / 73-25 6
Cy5 YES Long Pass 665 – 1200 2
Cy5 On Demand Single-band 725 / 150-25 On demand

Location

Contact Specialist

Usage Fee

10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

No documents available