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SYSTEM SPECIFICATIONS:
CAMERA SPECIFICATIONS:
| OBJECTIVES | ||||
| Objective | Magnification / NA | Medium | Contrast | Working Distance |
| EC Plan NeoFLUOR | 40x / 0.75 | water | – | 0.7 mm |
| EC Plan NeoFLUOR | 40x / 1.3 | oil | DIC | 0.2 mm |
| PL APOCHROMAT | 63x / 1.4 | oil | DIC | 0.18 mm |
| PLAN APO | 100x / 1.40 | oil | DIC | 0.17 mm |
| LASERLINES | |||
| FIXED wavelengths | Availability | Laser type | Some dyes and use |
| 405nm | YES | diode laser | DAPI |
| 440 nm | YES | diode laser | CyanFP |
| 488nm | YES | diode laser | GFP, Alexa488, CY2 |
| 514 nm | YES | diode laser | YellowFP |
| 555nm | YES | diode laser | RedFPs, Alexa568 |
| 639nm | YES | diode laser | DRAQ5, CY5, Alexa648 |
| FILTERCUBES for Zeiss stand | |||||
| Fluorochromes | Availability | Excitation | Dichroic | Emission | Filter No |
| DAPI | YES | 387 / 11-25 | 409 | 447 / 60-25 | DAPI-1160A |
| GFP | YES | 472 / 30-25 | 495 | 520 / 35-25 | GFP-3035 |
| CFP | YES | 438 / 24-25 | 458 | 483 / 32-25 | CFP-2432 |
| Cy3 | YES | 531 / 40-25 | 562 | 593 / 40-25 | Cy3-4040A |
| Cy5 | On Demand | 628 / 40-25 | 660 | 692 / 40-25 | Cy5-4040A |
| YFP | On Demand | 500 / 24-25 | 520 | 542 / 27-25 | YFP-2427A |
| BANDPASS FILTERS (Emitters) | ||||
| Fluorochromes | Availability | Bandpass | Emission | Position No |
| DAPI/FITC/Cy3/Cy5 | YES | Multi-band | 440 / 521 / 607 / 700-25 | 1 |
| DAPI | On Demand | Single-band | 445 / 45-25 | On Demand |
| CFP | YES | Single-band | 482 / 35-25 | 3 |
| GFP | YES | Single-band | 525 / 30-25 | 4 |
| YFP | YES | Single-band | 542 / 27-25 | 5 |
| RFP | YES | Single-band | 617 / 73-25 | 6 |
| Cy5 | YES | Long Pass | 665 – 1200 | 2 |
| Cy5 | On Demand | Single-band | 725 / 150-25 | On demand |
Room 184
Imaging Core Facility Biozentrum Basel
Switzerland
10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines
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A joint Network for scientific Imaging and Image Analysis Infrastructure
Phone: +49 761 203 2934
Email: info@miap.eu
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