3i Spinning Disk

3i spinning disk confocal "MARIANAS"

Equipment type: Light microscopy

csm_SystemSpecification_3iSpinDisk_ffc209b8b6
SYSTEM SPECIFICATIONS:
  • Imaging: Fast live imaging with low phototoxicity; FRAP; FRET; Photoconversion
  • Stand: Zeiss Axio Observer
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: Slidebook 5
CAMERA SPECIFICATIONS:
  • Model: Photometrics Evolve 512, back-illuminated EMCCD
  • Effective number of pixels: 512 x 512
  • Cell size: 16.0µm x 16.0µm
  • Effective Area: 8.0mm x 8.0mm
  • Max frame rate: 33.7 frames/second
  • Dynamic range: 16 bit
  • Quantum efficiency (peak): > 90%
  • Read out noise at min EM gain: 5 electrons
  • Read out noise at max EM gain: <0.5 electron
OBJECTIVES
ObjectiveMagnification / NAMediumContrastWorking Distance
EC Plan NeoFLUOR40x / 0.75water0.7 mm
EC Plan NeoFLUOR40x / 1.3oilDIC0.2 mm
PL APOCHROMAT63x / 1.4oilDIC0.18 mm
PLAN APO100x / 1.40oilDIC0.17 mm
LASERLINES
FIXED wavelengthsAvailabilityLaser typeSome dyes and use
405nmYESdiode laserDAPI
440 nmYESdiode laserCyanFP
488nmYESdiode laserGFP, Alexa488, CY2
514 nmYESdiode laserYellowFP
555nmYESdiode laserRedFPs, Alexa568
639nmYESdiode laserDRAQ5, CY5, Alexa648
FILTERCUBES for Zeiss stand
FluorochromesAvailabilityExcitationDichroicEmissionFilter No
DAPIYES387 / 11-25409447 / 60-25DAPI-1160A
GFPYES472 / 30-25495520 / 35-25GFP-3035
CFPYES438 / 24-25458483 / 32-25CFP-2432
Cy3YES531 / 40-25562593 / 40-25Cy3-4040A
Cy5On Demand628 / 40-25660692 / 40-25Cy5-4040A
YFPOn Demand500 / 24-25520542 / 27-25YFP-2427A
BANDPASS FILTERS (Emitters)
FluorochromesAvailabilityBandpassEmissionPosition No
DAPI/FITC/Cy3/Cy5YESMulti-band440 / 521 / 607 / 700-251
DAPIOn DemandSingle-band445 / 45-25On Demand
CFPYESSingle-band482 / 35-253
GFPYESSingle-band525 / 30-254
YFPYESSingle-band542 / 27-255
RFPYESSingle-band617 / 73-256
Cy5YESLong Pass665 – 12002
Cy5On DemandSingle-band725 / 150-25On demand

Location

Contact Specialist

Usage Fee

10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

No documents available