Nikon A1-FLIM

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Nikon A1R FLIM-FCS

Equipment type: Light microscopy

Motorized xy table + piezo z-stage

Galvano scanner, Resonant scanner

Lasers cw: 405, 457, 488, 514, 561, 640 nm
pulsed: 440 +-10nm, 485 +-10nm, 640 +-10nm (FLIM, FCS)
Detectors:
2 x PMTs
2 x Gasp detectors, 1 x Transmitted
32 channels spectral detector
2 x PMA40 (FLIM, FCS)
sCMOS Hamamatsu ORCA Flash4.0 (wide field, TIRF)
Incubator 37 C, CO2, Humidity (Okolab)

Funding via:

BIOSS

Location

Room -01.026 Signalhaus
Phone: +49 761 203-67542
Life Imaging Center
Germany

Contact Specialist

Life Imaging Center team
Phone: +49 761 203-2934
Email: lic@imaging.uni-freiburg.de

Usage Fee

30 € / hour
Special discounts might be applicable.
We may charge you for additional components.
For details check our usage fee overview or
the usage conditions document.

Documentation

Prerequisites for FLIM experiments
Turn on tutorial Nikon-A1-FLIM

Objectives

Filters

Cameras

Light Sources

Objectives

Pos.	Objective	Manufacturer No.	Mag.	N.A.	WD mm	Immersion	DIC	Int. No.
1	Plan Fluor 4x/0.13	MRH00041	4	0.13	17.2		–	265
2	Plan Fluor 10x/0.30 PH DLL	MRH10105	10	0.3	16.00		–	266
3	Plan Apo VC 20x/0.75	MRD70200	20	0.75	1.0		N2	267
4	Plan Apo 40x/0.95		40	0.95	0.14		M N2	268
5	Apo TIRF 60x/1.49 Oil Corr. 0.13-0.21	MRD01691	60	1.49	0.12	Oil	N2	269
6	Apo TIRF 100x/1.49 Oil Corr. 0.13-0.19MRD 01991	MRD01991	100	1.49	0.12	Oil	N2	270
A	Apo 40x/1.25 WI λ S Corr. 0.15-0.19		40	1.25	0.16-0.20	H2O	–	304

Filters

Pos.	Name	Excitation	DC/BS	Emission	Dye	Int. No.
1	F36-140 Laser	HC BP 390/40 BrightLine	Laser HC BS R405	BP 452/45 HC BrightLine	DAPI Laser	300
2	F36-148 Laser	HC BP 482/18 BrightLine	Laser HC BS R488 1 lambda PV flat	BP 525/45 HC BrightLine	GFP Laser	301
3	F36-156 Laser	HC BP 561/14 BrightLine	Laser HC BS R561	BP 609/54 HC BrightLine	RFP Laser	302
4	F36-163 Laser	HC BP 640/14 BrightLine	Laser HC BS R635	BP 676/29 HC BrightLine	Cy 5 Laser	303
5	DIC				DIC	304
stock	TRF89901	ZET 405/488/561/640x	ZT405/488/561/640rpc	ZET405/488/561/640m-TRF	DAPI+GFP+RFP+Cy 5	299
	GFP	BP 482/35	BS 488 LPXP	BP 550/49	GFP	339

Cameras

Light Sources

Model name	Type	Spectral range (nm)
Intensilight C-HGFIE	Metal Halide	340-700
NikonTi-E	Laser	405, 430, 440, 458, 488, 514, 561, 640

Microscopy and Image Analysis Platform (MIAP)

A joint Network for scientific Imaging and Image Analysis Infrastructure

Get in touch:

Phone: +49 761 203 2934
Email: info@miap.eu
Contact Form

How to find us:

Albert-Ludwigs Universität Freiburg
Signalling Campus Freiburg (SCF)
Habsburgerstr. 49
79104 Freiburg im Breisgau
Germany

Nikon A1-FLIM

Home / Nikon A1-FLIM

Nikon A1R FLIM-FCS

Equipment type: Light microscopy

Location

Contact Specialist

Usage Fee

Documentation

Microscopy and Image Analysis Platform (MIAP)

Get in touch:

How to find us:

© MIAP, University of Freiburg

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Nikon A1-FLIM

Home / Nikon A1-FLIM

Nikon A1R FLIM-FCS

Equipment type: Light microscopy

Location

Contact Specialist

Usage Fee

Documentation

Microscopy and Image Analysis Platform (MIAP)

Get in touch:

How to find us:

© MIAP, University of Freiburg

ImpressumPrivacy PolicyCookie Policy

Impressum
Privacy Policy
Cookie Policy