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SYSTEM SPECIFICATIONS:
CAMERA SPECIFICATIONS:
| OBJECTIVES | ||||
| Objective | Magnification / NA | Medium | Contrast | Working Distance |
| U Plan S Apo | 10x / 0.40 | air | – | 3.1 mm |
| U Plan S Apo | 20x / 0.75 | air | – | 0.6 mm |
| U Apo 340 | 40x / 1.35 | oil | – | 0.1 mm |
| Plan Apo N | 60x / 1.42 | oil | – | 0.15 mm |
| UPLS Apo | 100x / 1.40 | oil | – | 0.13 mm |
Note: Extra 1.6 magnification lens available on the Olympus stand
| SOLID STATE ILLUMINATION | |||
| Wavelengths | Availability | Laser type | Some dyes and use |
| 390 / 18 | YES | SSI | DAPI |
| 438 / 24 | YES | SSI | CyanFP |
| 475 / 28 | YES | SSI | GFP, Alexa488, CY2, FITC |
| 513 / 17 | YES | SSI | YellowFP |
| 542 / 27 | YES | SSI | TRITC |
| 575 / 25 | YES | SSI | mCherry, Alexa594 |
| 632 / 22 | YES | SSI | DRAQ5, CY5, Alexa639 |
| BANDPASS FILTERS (Emitters) + EYEPIECE FILTERS for Olympus stand | ||||
| Fluorochromes | Availability | Emission | Mirror Unit Position | Filter Wheel |
| DAPI | YES | 435 / 48 | Manually select 1 or 3 | “Standard” or “Alexa” |
| CFP | YES | 475 / 24 | Manually select 2 | “Live” |
| GFP/FITC | YES | 525 / 48 | Manually select 1 or 3 | “Standard”, “Alexa”, or “Live” |
| YFP | YES | 548 / 22 | Manually select 2 | “Live” |
| TRITC | YES | 597 / 45 | Manually select 1 | “Standard” |
| mCherry / Alexa594 | YES | 625 / 45 | Manually select 2 or 3 | “Live” or “Alexa” |
| Cy5 | YES | 679 / 34 | Manually select 1 or 3 | “Standard” or “Alexa” |
Note: All bandpass filters are single-band.
| MIRROR UNIT for Olympus stand (SELECT MANUALLY) | ||
| Fluorochromes | Availability | Position |
| DAPI / GFP / FITC / TRITC / Cy5 | YES | 1 |
| CFP / YFP | YES | 2 |
| DAPI / GFP / FITC / mCherry / Alexa594 / Cy5 | YES | 3 |
| DAPI / GFP / FITC / TRITC / mCherry / Alexa594 / Cy5 | YES | 6 |
Room 229
Imaging Core Facility Biozentrum Basel
Switzerland
8 CHF / hour (Mo-Fr 09:00 – 17:00)
4 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines
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