Deltavision Core

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Applied Precision widefield DV Core  

Equipment type: Light microscopy

SYSTEM SPECIFICATIONS:

  • Imaging: very fast and sensitive inverted widefield microscope
  • Stand: Olympus IX71
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: SoftWorx 4.1.2

CAMERA SPECIFICATIONS:

  • Model: Photometrics CoolSNAP HQ2, interline transfer CCD
  • Effective number of pixels: 1392 x 1040
  • Cell size: 6.45µm x 6.45µm
  • Effective Area: 8.98mm x 6.71mm
  • Max frame rate: 11 frames/second
  • Dynamic range: 14-bit
  • Quantum efficiency (peak): > 60%
  • Read out noise: 4-5 electrons
OBJECTIVES
Objective Magnification / NA Medium Contrast Working Distance
U Plan S Apo 10x / 0.40 air – 3.1 mm
U Plan S Apo 20x / 0.75 air – 0.6 mm
U Apo 340 40x / 1.35 oil – 0.1 mm
Plan Apo N 60x / 1.42 oil – 0.15 mm
UPLS Apo 100x / 1.40 oil – 0.13 mm

Note: Extra 1.6 magnification lens available on the Olympus stand

SOLID STATE ILLUMINATION
Wavelengths Availability Laser type Some dyes and use
390 / 18 YES SSI DAPI
438 / 24 YES SSI CyanFP
475 / 28 YES SSI GFP, Alexa488, CY2, FITC
513 / 17 YES SSI YellowFP
542 / 27 YES SSI TRITC
575 / 25 YES SSI mCherry, Alexa594
632 / 22 YES SSI DRAQ5, CY5, Alexa639
BANDPASS FILTERS (Emitters) + EYEPIECE FILTERS for Olympus stand
Fluorochromes Availability Emission Mirror Unit Position Filter Wheel
DAPI YES 435 / 48 Manually select 1 or 3 “Standard” or “Alexa”
CFP YES 475 / 24 Manually select 2 “Live”
GFP/FITC YES 525 / 48 Manually select 1 or 3 “Standard”, “Alexa”, or “Live”
YFP YES 548 / 22 Manually select 2 “Live”
TRITC YES 597 / 45 Manually select 1 “Standard”
mCherry / Alexa594 YES 625 / 45 Manually select 2 or 3 “Live” or “Alexa”
Cy5 YES 679 / 34 Manually select 1 or 3 “Standard” or “Alexa”

Note: All bandpass filters are single-band.

MIRROR UNIT for Olympus stand (SELECT MANUALLY)
Fluorochromes Availability Position
DAPI / GFP / FITC / TRITC / Cy5 YES 1
CFP / YFP YES 2
DAPI / GFP / FITC / mCherry / Alexa594 / Cy5 YES 3
DAPI / GFP / FITC / TRITC / mCherry / Alexa594 / Cy5 YES 6

Equipment Booking

Location

Room 229
Imaging Core Facility Biozentrum Basel
Switzerland

Contact Specialist

Dr. Oliver Biehlmaier
Phone: +41 61207 2073
Email: oliver.biehlmaier@unibas.ch

Usage Fee

8 CHF / hour (Mo-Fr 09:00 – 17:00)
4 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

No documents available

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Email: info@miap.eu
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Germany

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