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FEI More inverted widefield microscope

Equipment type: Light microscopy

SPECIFICATIONS:  
    • Imaging: very fast and stable inverted widefield microscope, FRAP, TIRF
    • Stand: MORE frame and enclosure
    • XY-stage: motorized
    • Fully equipped for live imaging, temperature and CO2 controlled
    • Software: Live Acquisition 2.5
    • Camera: Hamamatsu ORCA flash 4.0 cooled sCMOS 
      • Effective number of pixels: 2048 x 2048
      • Cell size: 6.5µm x 6.5µm
      • Effective Area: 13.312mm x 13.312mm
      • Maximum frame rate: 30fps
      • Dynamic range: 16-bit
      • Quantum efficiency (peak): > 70%
      • Read out noise: 1.9 electrons rms
       
 
OBJECTIVES
Objective Magnification / NA Medium Contrast Working Distance
U Plan S Apo 10x / 0.40 air PH2 3.1 mm
U Plan S Apo 40x / 0.95 air PH2 0.38 mm
TIRF APON 60x / 1.49 oil 0.1 mm
U Plan S Apo 100x / 1.4 oil 0.13 mm
ILLUMINATION
   Wavelengths Availability    Type Some dyes and use
405 YES Solid State Laser DAPI
488 YES Solid State Laser GFP, Alexa488, CY2, FITC
561 YES Solid State Laser TRITC
640 YES Solid State Laser DRAQ5, CY5, Alexa639
395/25 YES Solid State Light DAPI
434/17 YES   Solid State Light   CFP
470/24 YES   Solid State Light   GFP, Alexa488, CY2, FITC
510/10 YES   Solid State Light   YFP
550/15 YES   Solid State Light   TRITC, Cy3
575/25 YES   Solid State Light   on demand (mCherry)
631/28 YES   Solid State Light   DRAQ5, CY5, Alexa639
470 YES LED (Trans) Transillumination
540 YES LED (Trans) Transillumination
630 YES LED (Trans) Transillumination
BANDPASS FILTERS (Emitters)
Fluorochromes Availability Bandpass Transmission
DAPI YES single BP 434/17
GFP, Alexa488 YES single BP 517/20
RFP, Alexa568 YES single BP 590/20
Cy5, Alexa647 YES single BP 685/40
CFP/YFP YES dual BP 464/15 – 547/22
DAPI / GFP / Cy3 / Cy5 YES quad BP (LED) 432/20 – 515/18 – 595/19 – 730/75
DAPI / FITC / TRITC / Cy5 YES quad BP (Lasers) 390/40 – 528/48 – 609/37 – 683/40

Location

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Usage Fee

8 CHF / hour (Mo-Fr 09:00 – 17:00)
4 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

No documents available