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SPECIFICATIONS:
| OBJECTIVES | ||||
| Objective | Magnification / NA | Medium | Contrast | Working Distance |
| U Plan S Apo | 10x / 0.40 | air | PH2 | 3.1 mm |
| U Plan S Apo | 40x / 0.95 | air | PH2 | 0.38 mm |
| TIRF APON | 60x / 1.49 | oil | – | 0.1 mm |
| U Plan S Apo | 100x / 1.4 | oil | – | 0.13 mm |
| ILLUMINATION | |||
| Wavelengths | Availability | Type | Some dyes and use |
| 405 | YES | Solid State Laser | DAPI |
| 488 | YES | Solid State Laser | GFP, Alexa488, CY2, FITC |
| 561 | YES | Solid State Laser | TRITC |
| 640 | YES | Solid State Laser | DRAQ5, CY5, Alexa639 |
| 395/25 | YES | Solid State Light | DAPI |
| 434/17 | YES |
Solid State Light
|
CFP |
| 470/24 | YES |
Solid State Light
|
GFP, Alexa488, CY2, FITC |
| 510/10 | YES |
Solid State Light
|
YFP |
| 550/15 | YES |
Solid State Light
|
TRITC, Cy3 |
| 575/25 | YES |
Solid State Light
|
on demand (mCherry) |
| 631/28 | YES |
Solid State Light
|
DRAQ5, CY5, Alexa639 |
| 470 | YES | LED (Trans) | Transillumination |
| 540 | YES | LED (Trans) | Transillumination |
| 630 | YES | LED (Trans) | Transillumination |
| BANDPASS FILTERS (Emitters) | |||
| Fluorochromes | Availability | Bandpass | Transmission |
| DAPI | YES | single BP | 434/17 |
| GFP, Alexa488 | YES | single BP | 517/20 |
| RFP, Alexa568 | YES | single BP | 590/20 |
| Cy5, Alexa647 | YES | single BP | 685/40 |
| CFP/YFP | YES | dual BP | 464/15 – 547/22 |
| DAPI / GFP / Cy3 / Cy5 | YES | quad BP (LED) | 432/20 – 515/18 – 595/19 – 730/75 |
| DAPI / FITC / TRITC / Cy5 | YES | quad BP (Lasers) | 390/40 – 528/48 – 609/37 – 683/40 |
Room 183
Imaging Core Facility Biozentrum Basel
Switzerland
8 CHF / hour (Mo-Fr 09:00 – 17:00)
4 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines
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A joint Network for scientific Imaging and Image Analysis Infrastructure
Phone: +49 761 203 2934
Email: info@miap.eu
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