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Objectives
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| Model name | Type | Spectral range (nm) |
|---|---|---|
| HBO 50 | Mercury | 340-700 |
Upright confocal microscope (Leica) dedicated to the conventional confocal and multiphoton imaging of fixed and living samples, photoablation, SHG (on demand). Equipped with a climatic chamber (temperature control (RT to 37°C) only) and motorized x-y-z stages.
Fixed stage available (on demand) for intravital experiment.
| Â Illumination | Â Â Wavelength range, nm |
|---|---|
| Â Laser diode 405 nm | Â 405 |
| Â Argon laser | Â 458 476 488 496 514 |
| Â HeNe Laser 633 nm | Â 633 |
| Â Femtosecond Ti:Saphpulsing laser (Coherent) |
 690-1080 |
| Â Detection | Â Wavelength range, nm |
|---|---|
| Â 2 Internal (descanned) PMTs | Â 350 to 800 nm, adjustable spectral range |
| Â 2 Internal (descanned ) HyDs | Â 350 to 800 nm, adjustable spectral range |
| Â 2 Non-descanned trans PMTs | Â Default filter cubes: FITC/TRITC BP525/50-BS560-BP585/40On demand: 470/22 |
| Â 2 Non-descanned HyD | Â Default filter cubes: FITC/TXR BP525/50-BS560-BP617/73 |
| Â 1 PMT trans | Â Laser wavelength |
Software:
Leica LAS X software
– Acquisition modes: time lapse (xyt), z stack (xyz), spectral acquisitions (xy?), Multiposition  (Mark&Find), Overview Image (Tile Scan), Sequential acquisition
– Best Focus, FRAP and FRET modalities
– Processing tools for stitching, deconvolution, 3D visualization…
Room E1003 CBI
Imaging Center IGBMC
France
Imaging Center IGBMC team
Phone: +33 369485116
Email: guiote@igbmc.fr
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For more information see the
FacilityAccessCharter.pdf
For primary facility Access please see
The training form
External users need to fill the this form
Extern Access Form
For further questions please contact the
photonic team: groupe-mic-photon@igbmc.fr
No documents available
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