Leica SP8 MP

Point Scanning Confocal SP8 upright with multiphoton option

Equipment type: Light microscopy

Leica-SP8-MP

Upright confocal microscope (Leica) dedicated to the conventional confocal and multiphoton imaging of fixed and living samples, photoablation, SHG (on demand). Equipped with a climatic chamber (temperature control (RT to 37°C) only) and motorized x-y-z stages. Fixed stage available (on demand) for intravital experiment.

SPECIFICATIONS:

  Illumination    Wavelength range, nm
  Laser diode 405 nm  405
  Argon laser  458 476 488 496 514
  HeNe Laser 633 nm  633
  Femtosecond Ti:Saphpulsing laser (Coherent)  690-1080
 
  Detection  Wavelength range, nm
  2 Internal (descanned) PMTs  350 to 800 nm, adjustable spectral range
  2 Internal (descanned ) HyDs  350 to 800 nm, adjustable spectral range
  2 Non-descanned trans PMTs  Default filter cubes: FITC/TRITC BP525/50-BS560-BP585/40On demand: 470/22
  2 Non-descanned HyD  Default filter cubes: FITC/TXR BP525/50-BS560-BP617/73
  1 PMT trans  Laser wavelength

Software:

Leica LAS X software – Acquisition modes: time lapse (xyt), z stack (xyz), spectral acquisitions (xy?), Multiposition   (Mark&Find), Overview Image (Tile Scan), Sequential acquisition – Best Focus, FRAP and FRET modalities – Processing tools for stitching, deconvolution, 3D visualization…

Location

Room E1003 CBI
Imaging Center IGBMC
France

Contact Specialist

Imaging Center IGBMC team
Phone: +33 369485116
Email: guiote@igbmc.fr

Usage Fee

unknown

For more information see the
FacilityAccessCharter.pdf

For primary facility Access please see
The training form

External users need to fill the this form
Extern Access Form

For further questions please contact the
photonic team: groupe-mic-photon@igbmc.fr

Documentation

No documents available