Advanced Imaging Techniques (AIT 38)

Program

The main focus is on

• bright-field microscopy
• fluorescence microscopy
• time lapse microscopy with GFP und relatives for live cell and organelle-markers
• ratio-imaging with ion-sensitive dyes
• FRAP, FLIP, FRET, spectral unmixing and TIRF techniques
• photo-activation (uncaging) and photo-conversion techniques
• imaging in 2D, 3D, 4D and 5D
• software for image analysis and restoration, data presentation

In the morning lectures are dealing with theory, mechanics, and application of wide-field and fluorescent imaging methods are followed by practical software demonstrations. In the afternoon students work in small groups of three in extended laboratory practical’s on high end confocal or imaging microscope set-ups. Each group is coached by a specialist in microscopy. In the late afternoon and evening students work and analyze their data with high end computer workstations using state of the art image analysis and visualization software packages.

The attendees have to prepare a protocol of their work during the week. The protocols will be presented at the end of the course on Friday afternoon using Microsoft Power Point.