Imaris / Andor Workshop

Program

Imaris two-day Image Analysis Workshop (29.09.2022 – 30.09.2022) focuses on Image Data handling, Visualization and Analysis. The 1st day will deal with basic questions about image analysis tools and the 2nd day with questions for already experienced image analysts.

Beforehand (27.09.2022 – 28.09.2022) you can join an individual 1-hour hands-on training at the new benchtop confocal microscope BC43 with your samples.

Day 1 – Beginner Workshop (29.09.2022)

09:00 – 09:30 General introduction of Imaris
09:30 – 10:15 Import of data, preprocessing with hands-on
10:15 – 11:00 Visualization with hands-on
11:00 – 11:20 Coffee Break
11:20 – 12: 30 Surfaces and spots with hands-on

12:30 – 13:30 Lunch Break

13:30 – 14:30 Co-localisation object based and pixel based with hands-on
14:30 – 15:15 Cell with hands-on
15:15 – 15:30 Coffee Break
15:30 – 16:15 Filament with hands-on
16:15 – 17:00 Batch processing with hands-on

Day 2 – Advanced Workshop (30.09.2022)

9:00 – 9:30 Complex Animation (Objects and Channels)
9:30 – 10:15 Quantification  Hands-On

1. Intensity, Volume, Area, Count of Objects, Position
2. Plotting of Statistics
3. Filtering and Classification
4. Machine Learning object classification

10:15 – 11:00 Interaction Hands-On

5. Distances between objects
6. Spatial Distribution
7. Colocalisation of objects, Volume overlap
8. 3D Intensity Profiling

11:00-11:20 Break

11:20-12:00 Motion Analysis Hands-On

9. Cell division Tracking
10. Cell contacts over time
11. Event synchronization

12:00-13:00 Lunch Break

13:00-14:00 Batch Processing and Plotting Hands-On

12. Batch for Image Processing and Segmentation
13. Plotting of Batch Results
14. Comparison of experimental groups
15. Statistical Tests
16. Object interaction analysis

14:00-17:00 Open Space for image analysis

17. Questions
18. Working on own datasets