TissueVision

SYSTEM SPECIFICATIONS:

• • Imaging: Serial two-photon tomography
    • Whole organ imaging at high resolution without clearing procedures
    • No working distance limitations due to included microtome

     

  • Visualization of tissue with & without labeling by 2 photon microscopy
    • Auto fluorescence enables visualization of sample tissue and structures within the tissue due to differences in auto fluorescence emission spectra
    • Excitation of different fluorophores with one 2photon excitation wavelength and highly efficient emission detection with up to 3 non-descanned detectors.

     

  • Detection with 1664 x 1664 or 832 x 832 pixel/ frame
  • Typical x/y resolution: 1 to 2 microns per pixel. Typical z resolution:10 to 50 micronsThe software pipeline for:– Image reconstruction in MATLAB using StitchIt

    (https://github.com/BaselLaserMouse/StitchIt)

     

    – Visualisation of full resolution data sets using Arivis Vision-4D

    (https://www.arivis.com/en/imaging-science/arivis-vision4d)

    or the open-source tool, MASIV (https://github.com/alexanderbrown/masiv)is now available.

     

detection filters can be customized.