TissueVision

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TissueVision "TissueCyte"

Equipment type: Tomography

SYSTEM SPECIFICATIONS:  
    • Imaging: Serial two-photon tomography
      • Whole organ imaging at high resolution without clearing procedures
      • No working distance limitations due to included microtome
       
    • Visualization of tissue with & without labeling by 2 photon microscopy
      • Auto fluorescence enables visualization of sample tissue and structures within the tissue due to differences in auto fluorescence emission spectra
      • Excitation of different fluorophores with one 2photon excitation wavelength and highly efficient emission detection with up to 3 non-descanned detectors.
       
    • Detection with 1664 x 1664 or 832 x 832 pixel/ frame
    • Typical x/y resolution: 1 to 2 microns per pixel. Typical z resolution:10 to 50 micronsThe software pipeline for:– Image reconstruction in MATLAB using StitchIt (https://github.com/BaselLaserMouse/StitchIt)   – Visualisation of full resolution data sets using Arivis Vision-4D (https://www.arivis.com/en/imaging-science/arivis-vision4d) or the open-source tool, MASIV (https://github.com/alexanderbrown/masiv)is now available.  
 
  OBJECTIVES  
Objective Magnification / NA Dipping medium Use for Working Distance
Zeiss Plan Apo 20x / 1.0 Water Vis-IR 2.4mm
Zeiss Plan Apo 16x / 0.8 Water Vis-IR 3mm
   
  TUNABLE ULTRAFAST LASERS  
Type/Model Peak Power Pulse Width useful tuning Range Average Power Repetition rate
Mai Tai eHP > 450 kW < 70 fs 750 -960nm > 2.5 W 80 MHz
   
EMISSION FILTERS
Fluorochromes   670 nm multiphoton short-pass dichroic beam splitter Dichroic default detector filter
DAPI, CFP           SP 500
GFP / FITC / YFP LP 500
mCherry / Cy5 / A647 LP 560
  detection filters can be customized.

Location

Contact Specialist

Usage Fee

10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

No documents available