TissueVision

TissueVision "TissueCyte"

Equipment type: Tomography

csm_TC_Inside_edge_bda30eadbc_scale
SYSTEM SPECIFICATIONS:  
    • Imaging: Serial two-photon tomography
      • Whole organ imaging at high resolution without clearing procedures
      • No working distance limitations due to included microtome
       
    • Visualization of tissue with & without labeling by 2 photon microscopy
      • Auto fluorescence enables visualization of sample tissue and structures within the tissue due to differences in auto fluorescence emission spectra
      • Excitation of different fluorophores with one 2photon excitation wavelength and highly efficient emission detection with up to 3 non-descanned detectors.
       
    • Detection with 1664 x 1664 or 832 x 832 pixel/ frame
    • Typical x/y resolution: 1 to 2 microns per pixel. Typical z resolution:10 to 50 micronsThe software pipeline for:– Image reconstruction in MATLAB using StitchIt (https://github.com/BaselLaserMouse/StitchIt)   – Visualisation of full resolution data sets using Arivis Vision-4D (https://www.arivis.com/en/imaging-science/arivis-vision4d) or the open-source tool, MASIV (https://github.com/alexanderbrown/masiv)is now available.  
 
  OBJECTIVES  
ObjectiveMagnification / NADipping mediumUse forWorking Distance
Zeiss Plan Apo20x / 1.0WaterVis-IR2.4mm
Zeiss Plan Apo16x / 0.8WaterVis-IR3mm
   
  TUNABLE ULTRAFAST LASERS  
Type/ModelPeak PowerPulse Widthuseful tuning RangeAverage PowerRepetition rate
Mai Tai eHP> 450 kW< 70 fs750 -960nm> 2.5 W80 MHz
   
EMISSION FILTERS
Fluorochromes  670 nm multiphoton short-pass dichroic beam splitterDichroicdefault detector filter
DAPI, CFP          SP 500
GFP / FITC / YFPLP 500
mCherry / Cy5 / A647LP 560
  detection filters can be customized.

Location

Contact Specialist

Usage Fee

10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines

Documentation

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