Sutter Instrument Modular In vivo Multiphoton Microscopy System

Upright 2P laser scanning microscope Sutter MIMMS

• Tunable 2-photon laser
• Resonant scanner
• 2 GaAsP detectors
• 594nm 100 mW optogenetic stimulation, Rapp OptoElectronic UGA-42 Firefly
• Sutter micromanipulator positioning system
• Software: ScanImage Premium

Funding via:

DFG 91b; Prof. Dr. Andrew D. Straw

DFG Project No. 432704004

Objectives

16x 0.8NA 3mm Working Distance

Filters

Twitch:  Emissions: 480/40mm, 535/50

GCaMP + tdTomato: Emissions: 525/70m, 605/70m

Cameras

Photometrics Kinetix 22 sCMOS camera

Light Sources

Model name:  Coherent Chameleon Vision S

Type: Tunable Laser

Spectral range (nm): 690-1050

Model name: Rapp Optoelectronics KSL-2/5700K

Type: LED

Spectral range: White LED 5700K color temperature