Leica SP8

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Leica point scanning confocal “SP8“ inverted - BioSafety Level 2

Equipment type: Light microscopy

  • Imaging: Confocal for live imaging (L2)
  • Stand: Leica DMI 6000B with adaptive focus
  • XY-stage: motorized
  • Equipped with 1 HyD and 2 PMTs
  • Fully equipped for live imaging, temperature and CO2 controlled
  • AOBS serves as a tunable dichroic for all lasers
  • Software: LAS AF version 3
ObjectiveMagnification / NAMediumContrastAFCWorking Distance
HC PL Fluotar10x / 0.30airDICYes11 mm
HC PL APO CS20x / 0.70airDICYes0.59 mm
HC PL APO CS220x / 0.75IMMDICYes0.67 mm
HC PL APO CS40x / 1.10waterDICNo0.65 mm
HC PL APO CS263x / 1.40oilDICYes0.14 mm
FIXED wavelengthsAvailabilityLaser type and powerSome dyes and use
405nmYESContinuous wave laser, 50 mWDAPI, Hoechst
488nmYESSolid State laser, 20mWGFP, Alexa488, CY2, OregonGreen
552nmYESSolid State laser, 20mWRedFPs, Alexa568
638nmYESSolid State laser, 30mWDRAQ5, CY5, Alexa648
FluorochromesAvailabilityExcitationEmissionFilter No
Blue (DAPI, Hoechst)YESMulti-band470 / 40A4
Green (GFP)YESSingle-band525 / 50GFP
Red (TexasRed, Alexa568, Alexa594)YESSingle-band645 / 75TX2


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Usage Fee

10 CHF / hour (Mo-Fr 09:00 – 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines


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