FEI More

FEI More inverted widefield microscope

SPECIFICATIONS:

  • Imaging: very fast and stable inverted widefield microscope, FRAP, TIRF
  • Stand: MORE frame and enclosure
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: Live Acquisition 2.5
  • Camera: Hamamatsu ORCA flash 4.0 cooled sCMOS

    • Effective number of pixels: 2048 x 2048
    • Cell size: 6.5µm x 6.5µm
    • Effective Area: 13.312mm x 13.312mm
    • Maximum frame rate: 30fps
    • Dynamic range: 16-bit
    • Quantum efficiency (peak): > 70%
    • Read out noise: 1.9 electrons rms

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

U Plan S Apo

10x / 0.40

air

PH2

3.1 mm

U Plan S Apo

40x / 0.95

air

PH2

0.38 mm

TIRF APON

60x / 1.49

oil

-

0.1 mm

U Plan S Apo

100x / 1.4

oil

-

0.13 mm

ILLUMINATION

   Wavelengths

Availability

   Type

Some dyes and use

405

YES

Solid State Laser

DAPI

488

YES

Solid State Laser

GFP, Alexa488, CY2, FITC

561

YES

Solid State Laser

TRITC

640

YES

Solid State Laser

DRAQ5, CY5, Alexa639

395/25

YES

Solid State Light

DAPI

434/17

YES

Solid State Light

CFP

470/24

YES

Solid State Light

GFP, Alexa488, CY2, FITC

510/10

YES

Solid State Light

YFP

550/15

YES

Solid State Light

TRITC, Cy3

575/25

YES

Solid State Light

on demand (mCherry)

631/28

YES

Solid State Light

DRAQ5, CY5, Alexa639

470

YES

LED (Trans)

Transillumination

540

YES

LED (Trans)

Transillumination

630

YES

LED (Trans)

Transillumination

BANDPASS FILTERS (Emitters)

Fluorochromes

Availability

Bandpass

Transmission

DAPI

YES

single BP

434/17

GFP, Alexa488

YES

single BP

517/20

RFP, Alexa568

YES

single BP

590/20

Cy5, Alexa647

YES

single BP

685/40

CFP/YFP

YES

dual BP

464/15 - 547/22

DAPI / GFP / Cy3 / Cy5

YES

quad BP (LED)

432/20 - 515/18 - 595/19 - 730/75

DAPI / FITC / TRITC / Cy5

YES

quad BP (Lasers)

390/40 - 528/48 - 609/37 - 683/40

 

Location:

Room 183 
Imaging Core Facility (IMCF)
Biozentrum
Basel University

Contact Specialist:

Oliver Biehlmaier
Biozentrum, Basel University
Phone +41 61 207 20 73
E-Mail oliver.biehlmaier@unibas.ch

Usage Fee:

Price:
8 CHF / hour (Mo-Fr 09:00 - 17:00)
4 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the 
undefinedUsage guidelines

Documentation:

no documents available