FEI More
FEI More inverted widefield microscope
SPECIFICATIONS:
- Imaging: very fast and stable inverted widefield microscope, FRAP, TIRF
- Stand: MORE frame and enclosure
- XY-stage: motorized
- Fully equipped for live imaging, temperature and CO2 controlled
- Software: Live Acquisition 2.5
- Camera: Hamamatsu ORCA flash 4.0 cooled sCMOS
- Effective number of pixels: 2048 x 2048
- Cell size: 6.5µm x 6.5µm
- Effective Area: 13.312mm x 13.312mm
- Maximum frame rate: 30fps
- Dynamic range: 16-bit
- Quantum efficiency (peak): > 70%
- Read out noise: 1.9 electrons rms
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
U Plan S Apo | 10x / 0.40 | air | PH2 | 3.1 mm |
U Plan S Apo | 40x / 0.95 | air | PH2 | 0.38 mm |
TIRF APON | 60x / 1.49 | oil | - | 0.1 mm |
U Plan S Apo | 100x / 1.4 | oil | - | 0.13 mm |
ILLUMINATION | |||
Wavelengths | Availability | Type | Some dyes and use |
405 | YES | Solid State Laser | DAPI |
488 | YES | Solid State Laser | GFP, Alexa488, CY2, FITC |
561 | YES | Solid State Laser | TRITC |
640 | YES | Solid State Laser | DRAQ5, CY5, Alexa639 |
395/25 | YES | Solid State Light | DAPI |
434/17 | YES | Solid State Light | CFP |
470/24 | YES | Solid State Light | GFP, Alexa488, CY2, FITC |
510/10 | YES | Solid State Light | YFP |
550/15 | YES | Solid State Light | TRITC, Cy3 |
575/25 | YES | Solid State Light | on demand (mCherry) |
631/28 | YES | Solid State Light | DRAQ5, CY5, Alexa639 |
470 | YES | LED (Trans) | Transillumination |
540 | YES | LED (Trans) | Transillumination |
630 | YES | LED (Trans) | Transillumination |
BANDPASS FILTERS (Emitters) | |||
Fluorochromes | Availability | Bandpass | Transmission |
DAPI | YES | single BP | 434/17 |
GFP, Alexa488 | YES | single BP | 517/20 |
RFP, Alexa568 | YES | single BP | 590/20 |
Cy5, Alexa647 | YES | single BP | 685/40 |
CFP/YFP | YES | dual BP | 464/15 - 547/22 |
DAPI / GFP / Cy3 / Cy5 | YES | quad BP (LED) | 432/20 - 515/18 - 595/19 - 730/75 |
DAPI / FITC / TRITC / Cy5 | YES | quad BP (Lasers) | 390/40 - 528/48 - 609/37 - 683/40 |
Location:
Room 183
Imaging Core Facility (IMCF)
Biozentrum - Basel University
Contact Specialist:
Oliver Biehlmaier
Biozentrum, Basel University
+41 61 207 20 73
oliver.biehlmaier@unibas.ch
Usage Fee:
Price:
8 CHF / hour (Mo-Fr 09:00 - 17:00)
4 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines
Documentation:
no documents available