Deltavision Core

APPLIED PRECISION WIDEFIELD DV CORE

SYSTEM SPECIFICATIONS:

  • Imaging: very fast and sensitive inverted widefield microscope
  • Stand: Olympus IX71
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: SoftWorx 4.1.2

CAMERA SPECIFICATIONS:

  • Model: Photometrics CoolSNAP HQ2, interline transfer CCD
  • Effective number of pixels: 1392 x 1040
  • Cell size: 6.45µm x 6.45µm
  • Effective Area: 8.98mm x 6.71mm
  • Max frame rate: 11 frames/second
  • Dynamic range: 14-bit
  • Quantum efficiency (peak): > 60%
  • Read out noise: 4-5 electrons

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

U Plan S Apo

10x / 0.40

air

-

3.1 mm

U Plan S Apo

20x / 0.75

air

-

0.6 mm

U Apo 340

40x / 1.35

oil

-

0.1 mm

Plan Apo N

60x / 1.42

oil

-

0.15 mm

UPLS Apo

100x / 1.40

oil

-

0.13 mm

Note: Extra 1.6 magnification lens available on the Olympus stand

SOLID STATE ILLUMINATION

Wavelengths

Availability

Laser type

Some dyes and use

390 / 18

YES

SSI

DAPI

438 / 24

YES

SSI

CyanFP

475 / 28

YES

SSI

GFP, Alexa488, CY2, FITC

513 / 17

YES

SSI

YellowFP

542 / 27

YES

SSI

TRITC

575 / 25

YES

SSI

mCherry, Alexa594

632 / 22

YES

SSI

DRAQ5, CY5, Alexa639

BANDPASS FILTERS (Emitters) + EYEPIECE FILTERS for Olympus stand

Fluorochromes

Availability

Emission

Mirror Unit Position

Filter Wheel

DAPI

YES

435 / 48

Manually select 1 or 3

“Standard” or “Alexa”

CFP

YES

475 / 24

Manually select 2

“Live”

GFP/FITC

YES

525 / 48

Manually select 1 or 3

“Standard”, “Alexa”, or “Live”

YFP

YES

548 / 22

Manually select 2

“Live”

TRITC

YES

597 / 45

Manually select 1

“Standard”

mCherry / Alexa594

YES

625 / 45

Manually select 2 or 3

“Live” or “Alexa”

Cy5

YES

679 / 34

Manually select 1 or 3

“Standard” or “Alexa”

NoteAll bandpass filters are single-band.

MIRROR UNIT for Olympus stand (SELECT MANUALLY)

Fluorochromes

Availability

Position

DAPI / GFP / FITC / TRITC / Cy5

YES

1

CFP / YFP

YES

2

DAPI / GFP / FITC / mCherry / Alexa594 / Cy5

YES

3

DAPI / GFP / FITC / TRITC / mCherry / Alexa594 / Cy5

YES

6

Location:

Room 229 
Imaging Core Facility (IMCF)
Biozentrum
Basel University

Contact Specialist:

Oliver Biehlmaier
Biozentrum, Basel University
Phone +41 61 207 20 73
E-Mail oliver.biehlmaier@unibas.ch

Usage Fee:

Price:
8 CHF / hour (Mo-Fr 09:00 - 17:00)
4 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the 
undefinedUsage guidelines

Documentation:

no documents available