TissueVision

TissueVision "TissueCyte"

SYSTEM SPECIFICATIONS:

  • Imaging: Serial two-photon tomography

    • Whole organ imaging at high resolution without clearing procedures
    • No working distance limitations due to included microtome

  • Visualization of tissue with & without labeling by 2 photon microscopy

    • Auto fluorescence enables visualization of sample tissue and structures within the tissue due to differences in auto fluorescence emission spectra
    • Excitation of different fluorophores with one 2photon excitation wavelength and highly efficient emission detection with up to 3 non-descanned detectors.

  • Detection with 1664 x 1664 or 832 x 832 pixel/ frame
  • Typical x/y resolution: 1 to 2 microns per pixel. Typical z resolution:10 to 50 microns

    The software pipeline for:

    - Image reconstruction in MATLAB using StitchIt

    (https://github.com/BaselLaserMouse/StitchIt)

    - Visualisation of full resolution data sets using Arivis Vision-4D

    (https://www.arivis.com/en/imaging-science/arivis-vision4d)

    or the open-source tool, MASIV (https://github.com/alexanderbrown/masiv)

    is now available.

     

 

OBJECTIVES

Objective

Magnification / NA

Dipping medium

Use for

Working Distance

Zeiss Plan Apo

20x / 1.0

Water

Vis-IR

2.4mm

Zeiss Plan Apo

16x / 0.8

Water

Vis-IR

3mm

 

 

 

TUNABLE ULTRAFAST LASERS

Type/Model

Peak Power

Pulse Width

useful tuning Range

Average Power

Repetition rate

Mai Tai eHP

> 450 kW

< 70 fs

750 -960nm

> 2.5 W

80 MHz

 

 


EMISSION FILTERS

Fluorochromes

 

670 nm multiphoton short-pass dichroic beam splitter

Dichroic

default detector filter

DAPI, CFP

          SP 500

---

GFP / FITC / YFP

LP 500

---

mCherry / Cy5 / A647

LP 560

---

 

 detection filters can be customized.

Location:

Room 182
Imaging Core Facility (IMCF)
Biozentrum
Basel University

Contact Specialist:

Oliver Biehlmaier
Biozentrum, Basel University
Phone +41 61 207 20 73
E-Mail oliver.biehlmaier@unibas.ch

Usage Fee:

Price:
10 CHF / hour (Mo-Fr 09:00 - 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the 
Usage guidelines

Documentation:

no documents available