Imaging Core Facility (IMCF) - Biozentrum

SYSTEM SPECIFICATIONS:

• Imaging: Serial two-photon tomography
  • Whole organ imaging at high resolution without clearing procedures
  • No working distance limitations due to included microtome

• Visualization of tissue with & without labeling by 2 photon microscopy
  
  • Auto fluorescence enables visualization of sample tissue and structures within the tissue due to differences in auto fluorescence emission spectra
  • Excitation of different fluorophores with one 2photon excitation wavelength and highly efficient emission detection with up to 3 non-descanned detectors.

• Detection with 1664 x 1664 or 832 x 832 pixel/ frame

• Typical x/y resolution: 1 to 2 microns per pixel. Typical z resolution:10 to 50 microns

  The software pipeline for:

  - Image reconstruction in MATLAB using StitchIt

  (https://github.com/BaselLaserMouse/StitchIt)

  - Visualisation of full resolution data sets using Arivis Vision-4D

  (https://www.arivis.com/en/imaging-science/arivis-vision4d)

  or the open-source tool, MASIV (https://github.com/alexanderbrown/masiv)

  is now available.

   

 detection filters can be customized.