OMX Blaze
APPLIED PRECISION "OMX BLAZE" Super-Resolution
SYSTEM SPECIFICATIONS:
- Imaging: Super-resolution (fixed and live), structured illumination (3D-SIM), Ring TIRF (works with PALM or TIRF techniques)
- SIM resolution xy 100-160 nm; z 280-350 nm – Pixel resolution after reconstruction = 41 nm
- Stand: DeltaVision OMX Optical Microscope (version 4)
- XYZ-stage: motorized with 25 mm travel maximum. Piezo controlled Z axis system
- UltimateFocus Hardware Autofocus module with FocusAssist
- Fully equipped for live imaging, temperature and CO2 controlled
- Software: DeltaVision OMX softWoRx
CAMERA SPECIFICATIONS:
- 4 liquid cooled sCMOS cameras
- Effective number of pixels: 512 x 512 for SIM imaging, 1024 x 1024 for widefield
- Resolution: 0.08 µm pixel size
- Total chip size: 5.5 Megapixels
- Max frame rate: 400 frames/second
- Rolling Shutter and Global Shutter modes
- Dynamic range: 15 bit readout
- Quantum efficiency (peak): ~ 65%
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Use for | Working Distance |
U-PLAN S-APO | 100x / 1.40 | oil | Widefield + DIC | 0.12 mm |
Plan Apo N | 60x / 1.42 | oil | 3D-SIM | 0.15 mm |
APON | 60x / 1.49 | oil | TIRF | 0.1 mm |
UPLS Apo | 60x / 1.20 | water | (to be delivered) | 0.28 mm |
LASERLINES (SOLID STATE ILLUMINATION) | |||
FIX wavelengths (nm) | Availability | Laser type | Some dyes and use |
405 | YES | InsightSSI | DAPI |
445 | YES | InsightSSI | CyanFP |
488 | YES | InsightSSI | GFP, Alexa488, CY2 |
514 | YES | InsightSSI | YellowFP |
568 | YES | InsightSSI | RedFPs, Alexa568 |
642 | YES | InsightSSI | DRAQ5, CY5, Alexa647 |
EMISSION FILTERS | ||||
Fluorochromes | Availability | Bandpass | Emission | Drawer |
DAPI | YES | Single-band | 435 / 31 | BGR |
CFP | YES | Single-band | 477 / 35 | CYR |
GFP / FITC | YES | Single-band | 528 / 48 | BGR |
YFP | YES | Single-band | 541 / 22 | CYR |
mCherry / Alexa568 | YES | Single-band | 609 / 37 | BGR / CYR |
Cy5 / Alexa647 | YES | Single-band | 683 / 40 | BGR |
Location:
Room 185
Imaging Core Facility (IMCF)
Biozentrum - Basel University
Contact Specialist:
Oliver Biehlmaier
Biozentrum, Basel University
+41 61 207 20 73
oliver.biehlmaier@unibas.ch
Usage Fee:
Price:
10 CHF / hour (Mo-Fr 09:00 - 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines
Documentation:
no documents available