External 2-P Microscopes outside MIAP:
2-P microscopes feature multiple benefits. They allow to overcome penetration issues in larger samples and at the same time using higher wavelength than regular confocal systems, therefore reducing damaging effects on tissue and fluorescent labels. Within the last years, many workgroups at the University of Freiburg and the Universitätsklinikum (Clinics) have invested in latest 2-P microscopy technology. With specific scientific and clinical questions in mind, each workgroup listed below is running a 2-P microscope system of distinct flavor and extensions.
The following workgroups at the University of Freiburg or the Universitätsklinikum (Clinics) are no official members of MIAP but are closely collaborating with MIAP facilities on a regular basis (research, training, workshops). They also use MIAP infrastructure for numerous research projects.
List of external workgroups with 2-P microscopy systems (alphabetical order):
AG Prof. Dr. Marlene Bartos
How is information processed and encoded in neuronal networks to realize learning, memory and behaviour? This is one of the most fundamental questions in modern life sciences. We aim to uncover the mechanisms underlying information processing by applying electrophysiological, imaging, molecular and computational approaches.
AG Dr. Johann Bollmann
Sensory-controlled, goal-directed motor sequences are crucial for the survival of an animal. We aim at identifying fundamental neural mechanisms that enable an animal to rapidly analyze its sensory environment, to make behavioral decisions and to generate sequences of coordinated motor patterns. We use zebrafish as a neurobiological model for studying how these complex neural processing tasks are implemented in the vertebrate central nervous system. Our tools are multiphoton calcium imaging, single-cell recordings targeted at genetically defined neuronal cell types and analysis of synaptic connectivity from 3D electron microscopy.
AG Prof. Dr. Ilka Diester
The ability to move is a fundamental feature of most animals which allows them to actively interact with our environment. We are investigating the underlying neural mechanisms and circuits of this ability. We do so with electrophysiological recordings and optogenetic manipulations combined with behavioral analysis in rodents. We look into the local processing of movement preparation and generation in the motor cortex as well as higher order structures, e.g. prefrontal cortex. The goal is to create a better understanding of how neural subpopulations and pathways within and across brain areas influence motor behavior. In order to address these scientific aims we are constantly working on improving the existing techniques. We currently focus on the design of new optoelectronic probes and targeting strategies. Apart from advancing our basic knowledge about the neural mechanisms of movements, our results might help improving the design of new prosthetic devices and understanding of disorders in which the normal production of movements is disrupted.
AG Prof. Dr. Wolfgang Driever
Research in the laboratory focuses on the analysis of developmental mechanisms at the molecular level. We use the zebrafish as model system to combine genetics, genomics, signaling research, experimental embryology and optogenetics to achieve a quantitative understanding of complex signaling and regulatory networks in development. One focus is on nervous system development, aiming to understand neuronal differentiation as well as specific aspects of circuit formation and function.
AG Prof. Dr. Bernd Fakler
At the Institute of Physiology, Prof. Bernd Fakler heads a multi-disciplinary team of scientists with expertise in biochemistry, molecular biology, electrophysiology, mass spectrometry, structural biology and bioinformatics. Our central goal is comprehensive understanding of organization and operation of rapid signal transduction and information processing at the plasma membrane of excitable cells under normal and pathophysiological conditions.
AG Prof. Dr. Peter Kohl
The new Institute for Experimental Cardiovascular Medicine, directed by Prof Peter Kohl, forms one of five structural units comprising the University Heart Centre (UHZ) of the Medical Centre – University of Freiburg. The institute, a hub of interdisciplinary collaboration and translational research, is based at a newly-built research block, with modern research infrastructure and state-of-the-art laboratories. To facilitate interactions with theoretical, technical, biological and medical research, it is built on columns of technical expertise, rather than structured to target a specific level or topic of investigation.
AG Prof. Dr. Meyer-Luehmann
Aggregation and accumulation of proteins in the brain are common features of diverse age-related neurodegenerative diseases. Each of these neurodegenerative diseases is associated with abnormalities in the folding of a different protein leading to protein aggregation and ultimately to neuronal death. Alzheimers Disease (AD) is one of these protein conformational diseases which is characterized by the extracellular accumulation of amyloid-? peptide and neuroanatomical changes. Kinetic studies have shown that A? protein aggregation occur via a nucleation mechanism, which resembles a crystallization process (Figure 1). However, little is known about how protein aggregation and deposition is initiated in vivo.
AG Prof. Dr. Marco Prinz
Our group focuses on the role of the brain specific innate immune system. Important molecules involved in innate immunity are chemokine receptors, Toll-like receptors (TLRs) and cytokines such as interferons. Major player of this system within the brain are brain macrophages (microglia) which serve as the first barrier for invading pathogens. We are currently investigating the mechanisms by which microglia contribute to the induction and resolution of brain damage using mouse models of multiple sclerosis (EAE, cuprizone model) and neurodegeneration, e.g. Alzheimers disease.
AG Prof. Dr. Dierk Reiff
Our lab is interested in visual information processing and the control of visually guided behaviour in fruit flies (Drosophila melanogaster). Small animals like fruit flies exhibit stunningly complex and robust innate behaviours that warrant survival and reproduction of the animal. Thereby, behaviour is controlled by neural circuitries of small size and aparantly apparently reduced complexity. About 100.000 neurons, that may fall into maximallyum 150 different neuronal classes, establish the connections and the highly repetitive micro-circuitries of the Drosophila visual system. Some of these neurons and circuitries are specialized in extracting and computing information on visual motion and colour from the visual scene. Identification and characterization of these neurons is at the focus of research in our lab.
AG Prof. Dr. Andrew Straw
The lab of Andrew Straw studies neural circuits and behavior at the University of Freiburg, Germany. By developing advanced technical systems such as virtual reality arenas, they investigate the mechanistic basis of visual behaviors such as navigation in Drosophila: Neural circuits for vision; Engineering tools for quantitative behavior; Mapping neural circuits for visual locomotor guidance in Drosophila; How flies resolve conflicting visual information – cells and models