Leica SP8 MP

Leica Point Scanning Confocal SP8 upright with multiphoton option

Upright confocal microscope (Leica) dedicated to the conventional confocal and multiphoton imaging of fixed and living samples, photoablation, SHG (on demand). Equipped with a climatic chamber (temperature control (RT to 37°C) only) and motorized x-y-z stages.
Fixed stage available (on demand) for intravital experiment.

SPECIFICATIONS:

  Illumination      Wavelength range, nm   
  Laser diode 405 nm    405 
  Argon laser    458 476 488 496 514 
  HeNe Laser 633 nm    633 
  Femtosecond Ti:Saphpulsing laser 
  (Coherent) 		   690-1080 

 

  Detection    Wavelength range, nm 
  2 Internal (descanned) PMTs    350 to 800 nm, adjustable spectral range 
  2 Internal (descanned ) HyDs    350 to 800 nm, adjustable spectral range 
  2 Non-descanned trans PMTs 

  Default filter cubes: FITC/TRITC 
  BP525/50-BS560-BP585/40 

  On demand: 
  470/22 
  2 Non-descanned HyD    Default filter cubes: FITC/TXR 
  BP525/50-BS560-BP617/73 
  1 PMT trans    Laser wavelength 

 

Software:

Leica LAS X software
- Acquisition modes: time lapse (xyt), z stack (xyz), spectral acquisitions (xy?), Multiposition   (Mark&Find), Overview Image (Tile Scan), Sequential acquisition
- Best Focus, FRAP and FRET modalities 
- Processing tools for stitching, deconvolution, 3D visualization…

 

Microscope Booking (IGMBC intranet only)

Location:

Room: E1003 CBI
Imaging Center IGBMC (ICI)
IGBMC - Illkirch-Graffenstaden
 

Contact Specialist:

Team Microscopie Photonique
Imaging Center IGBMC (ICI)
Phone: +33 36948 5116
Mail: groupe-mic-photon@igbmc.fr

Usage Fee:

Price: unknown

For more information see the
FacilityAccessCharter.pdf

For primary facility Access please see
The training form

External users need to fill the this form
Extern Access Form

For further questions please contact the
photonic team:groupe-mic-photon@igbmc.fr