Perkin Elmer Spinning Disk

PERKIN ELMER SPINNING DISK CONFOCAL "ULTRA VIEW VoX"

SYSTEM SPECIFICATIONS:

    • Imaging: Fast live imaging with low phototoxicity; FRAP
    • Stand: Leica DMI 6000B with adaptive focus control
    • XY-stage: motorized
    • Fully equipped for live imaging, temperature and CO2 controlled
    • Photokinesis (FRAP) unit
    • Software: Volocity

      CAMERA SPECIFICATIONS:

      • Model: two Hamamatsu C9100-50 frame transfer EMCCD cameras
      • Effective number of pixels: 1000 x 1000
      • Cell size: 8.0µm x 8.0µm
      • Effective Area: 8.0mm x 8.0mm
      • Max frame rate: 28.1 frames/second
      • Dynamic range: 14 bit
      • Quantum efficiency (peak): > 60%
      • Read out noise at min EM gain: 10 electrons
      • Read out noise at max EM gain: <1 electron

      TWO ACQUISITION MODES AVAILABLE:

      1. SINGLE camera setup
      2. DUAL camera setup

      OBJECTIVES

      Objective

      Magnification / NA

      Medium

      Working Distance

      HC PLAN APO

      10x / 0.40

      IMM

      0.36 mm

      HC PLAN APO

      20x / 0.70

      IMM

      0.26 mm

      HXC PLAN S APO

      40x / 1.25-0.75

      oil

      0.1 mm

      HXC PLAN S APO

      63x / 1.40-0.60

      oil

      0.1 mm

      U PLAN S APO

      100x / 1.40-0.70

      oil

      0.09 mm

      HXC PLAN S APO

      63x / 1.20

      water

      0.22 mm

      LASERLINES

      FIXED wavelengths

      Availability

      Laser type and power

      Some dyes and use

      405nm

      YES

      solid state laser, 50 mW

      DAPI

      445nm*

      YES

      solid state laser, 40 mW

      CyanFP

      488nm

      YES

      solid state laser, 50 mW

      GFP, Alexa488, CY2, OregonGreen

      514nm*

      YES

      solid state laser, 25 mW

      YellowFP

      561nm

      YES

      solid state laser, 50 mW

      RedFPs, Alexa568

      640nm

      YES

      solid state laser, 40 mW

      DRAQ5, CY5, Alexa648

         *CFP/YFP imaging is available only on request.

       

      DICHROICS

      Dichroic

      Availability

      Extra Emission Filter 1

      Extra Emission Filter 2

      Kit No

      580 nm edge

      YES

      dual band 594/42-730/140

      dual band 448/55-523/47

      L7268950

      509 nm edge

      On demand

      dual band 594/42-730/140

      475/42

      L7268951

      BANDPASS FILTERS (Emitters)

      Fluorochromes

      Availability

      Bandpass

      Emission

      Em wheel No

      DAPI / RFP

      YES

      Dual-band

      445 / 60-615 / 70

      Set 2

      CFP / Far-Red

      YES

      Dual-band

      485 / 60-705 / 90

      Set 2

      GFP

      YES

      Single-band

      525 / 50

      Set 2

      YFP

      YES

      Single-band

      550 / 49

      Set 2

       

      Location:

      Room 187
      Imaging Core Facility (IMCF)
      Biozentrum
      Basel University

      Contact Specialist:

      Oliver Biehlmaier
      Biozentrum, Basel University
      Phone +41 61 207 20 73
      E-Mail oliver.biehlmaier@unibas.ch

      Usage Fee:

      Price:
      10 CHF / hour (Mo-Fr 09:00 - 17:00)
      5 CHF / hour (night and weekend)
      This is for your information only. MIAP
      is not responsible for correct usage fee
      information. For details and legally
      binding price information check the 
      undefinedUsage guidelines

      Documentation:

      no documents available