Leica SP8

Leica Point Scanning Confocal “SP8“ inverted - BioSafety Level 2

SPECIFICATIONS:

  • Imaging: Confocal for live imaging (L2)
  • Stand: Leica DMI 6000B with adaptive focus
  • XY-stage: motorized
  • Equipped with 1 HyD and 2 PMTs
  • Fully equipped for live imaging, temperature and CO2 controlled
  • AOBS serves as a tunable dichroic for all lasers
  • Software: LAS AF version 3

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

AFC

Working Distance

HC PL Fluotar

10x / 0.30

air

DIC

Yes

11 mm

HC PL APO CS

20x / 0.70

air

DIC

Yes

0.59 mm

HC PL APO CS2

20x / 0.75

IMM

DIC

Yes

0.67 mm

HC PL APO CS

40x / 1.10

water

DIC

No

0.65 mm

HC PL APO CS2

63x / 1.40

oil

DIC

Yes

0.14 mm

LASERLINES

FIXED wavelengths

Availability

Laser type and power

Some dyes and use

405nm

YES

Continuous wave laser, 50 mW

DAPI, Hoechst

488nm

YES

Solid State laser, 20mW

GFP, Alexa488, CY2, OregonGreen

552nm

YES

Solid State laser, 20mW

RedFPs, Alexa568

638nm

YES

Solid State laser, 30mW

DRAQ5, CY5, Alexa648

FILTERCUBES

Fluorochromes

Availability

Excitation

Emission

Filter No

Blue (DAPI, Hoechst)

YES

Multi-band

470 / 40

A4

Green (GFP)

YES

Single-band

525 / 50

GFP

Red (TexasRed, Alexa568, Alexa594)

YES

Single-band

645 / 75

TX2

Location:

Room 448 
Imaging Core Facility (IMCF)
Biozentrum
Basel University

Contact Specialist:

Oliver Biehlmaier
Biozentrum, Basel University
Phone +41 61 207 20 73
E-Mail oliver.biehlmaier@unibas.ch

Usage Fee:

Price:
10 CHF / hour (Mo-Fr 09:00 - 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the 
undefinedUsage guidelines

Documentation:

no documents available