Leica SP8
Leica Point Scanning Confocal “SP8“ inverted - BioSafety Level 2
SPECIFICATIONS:
- Imaging: Confocal for live imaging (L2)
- Stand: Leica DMI 6000B with adaptive focus
- XY-stage: motorized
- Equipped with 1 HyD and 2 PMTs
- Fully equipped for live imaging, temperature and CO2 controlled
- AOBS serves as a tunable dichroic for all lasers
- Software: LAS AF version 3
OBJECTIVES | |||||
Objective | Magnification / NA | Medium | Contrast | AFC | Working Distance |
HC PL Fluotar | 10x / 0.30 | air | DIC | Yes | 11 mm |
HC PL APO CS | 20x / 0.70 | air | DIC | Yes | 0.59 mm |
HC PL APO CS2 | 20x / 0.75 | IMM | DIC | Yes | 0.67 mm |
HC PL APO CS | 40x / 1.10 | water | DIC | No | 0.65 mm |
HC PL APO CS2 | 63x / 1.40 | oil | DIC | Yes | 0.14 mm |
LASERLINES | |||
FIXED wavelengths | Availability | Laser type and power | Some dyes and use |
405nm | YES | Continuous wave laser, 50 mW | DAPI, Hoechst |
488nm | YES | Solid State laser, 20mW | GFP, Alexa488, CY2, OregonGreen |
552nm | YES | Solid State laser, 20mW | RedFPs, Alexa568 |
638nm | YES | Solid State laser, 30mW | DRAQ5, CY5, Alexa648 |
FILTERCUBES | ||||
Fluorochromes | Availability | Excitation | Emission | Filter No |
Blue (DAPI, Hoechst) | YES | Multi-band | 470 / 40 | A4 |
Green (GFP) | YES | Single-band | 525 / 50 | GFP |
Red (TexasRed, Alexa568, Alexa594) | YES | Single-band | 645 / 75 | TX2 |
Location:
Room 448
Imaging Core Facility (IMCF)
Biozentrum - Basel University
Contact Specialist:
Oliver Biehlmaier
Biozentrum, Basel University
+41 61 207 20 73
oliver.biehlmaier@unibas.ch
Usage Fee:
Price:
10 CHF / hour (Mo-Fr 09:00 - 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the
Usage guidelines
Documentation:
no documents available