Leica SP5 II Matrix

LEICA POINT SCANNING CONFOCAL “SP5-II-MATRIX“ inverted

SPECIFICATIONS:

  • Imaging: Confocal for live imaging; FRAP
  • Stand: Leica DMI 6000B with adaptive focus
  • XY-stage: motorized
  • Equipped with 2 HyD
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Conventional scanner and resonant (ultra-fast, for reduced photodamage) scanner
  • AOBS serves as a tunable dichroic for all lasers
  • Software: LAS AF version 2.6.0.7266

OBJECTIVES

Objective

Magnification / NA

Medium

Availability

AFC

Working Distance

HC PL Fluotar

10x / 0.30

air

Yes

Yes

11 mm

HCX IRAPO L

25x / 0.95

water

On Demand

No

2.40 mm

HCX PLAN APO CS

40x / 1.10

water

Yes

No

0.63 mm

HCX PLAN APO

63x / 1.20

water

Yes

No

0.22 mm

HCX PLAN APO lambda blue

63x / 1.40-0.60

oil

Yes

Yes

0.10 mm

HCX PLAN APO lambda blue

40x / 1.25

oil

Yes

Yes

0.10 mm

HCX PLAN APO CS

20x / 0.70

air

Yes

Yes

0.59 mm

LASERLINES

FIXED wavelengths

Availability

Laser type and power

Some dyes and use

405nm

YES

diode laser, 50 mW

DAPI

458nm

YES

Argon laser, 5 mW

CyanFP

476nm

YES

Argon laser, 5 mW

 

488nm

YES

Argon laser, 20 mW

GFP, Alexa488, CY2, OregonGreen

496nm

YES

Argon laser, 5 mW

 

514nm

YES

Argon laser, 20 mW

YellowFP

561nm

YES

DPSS laser, 20 mW

RedFPs, Alexa568

633nm

YES

HeNe laser, 10 mW

DRAQ5, CY5, Alexa648

Location:

Room 187 
Imaging Core Facility (IMCF)
Biozentrum
Basel University

Contact Specialist:

Oliver Biehlmaier
Biozentrum, Basel University
Phone +41 61 207 20 73
E-Mail oliver.biehlmaier@unibas.ch

Usage Fee:

Price:
10 CHF / hour (Mo-Fr 09:00 - 17:00)
5 CHF / hour (night and weekend)
This is for your information only. MIAP
is not responsible for correct usage fee
information. For details and legally
binding price information check the 
undefinedUsage guidelines

Documentation:

no documents available