3i Spinning Disk

3i SPINNING DISK CONFOCAL "MARIANAS"

SYSTEM SPECIFICATIONS:

    • Imaging: Fast live imaging with low phototoxicity; FRAP; FRET; Photoconversion
    • Stand: Zeiss Axio Observer
    • XY-stage: motorized
    • Fully equipped for live imaging, temperature and CO2 controlled
    • Software: Slidebook 5

      CAMERA SPECIFICATIONS:

      • Model: Photometrics Evolve 512, back-illuminated EMCCD
      • Effective number of pixels: 512 x 512
      • Cell size: 16.0µm x 16.0µm
      • Effective Area: 8.0mm x 8.0mm
      • Max frame rate: 33.7 frames/second
      • Dynamic range: 16 bit
      • Quantum efficiency (peak): > 90%
      • Read out noise at min EM gain: 5 electrons
      • Read out noise at max EM gain: <0.5 electron

      OBJECTIVES

      Objective

      Magnification / NA

      Medium

      Contrast

      Working Distance

      EC Plan NeoFLUOR

      40x / 0.75

      water

      -

      0.7 mm

      EC Plan NeoFLUOR

      40x / 1.3

      oil

      DIC

      0.2 mm

      PL APOCHROMAT

      63x / 1.4

      oil

      DIC

      0.18 mm

      PLAN APO

      100x / 1.40

      oil

      DIC

      0.17 mm

      LASERLINES

      FIXED wavelengths

      Availability

      Laser type

      Some dyes and use

      405nm

      YES

      diode laser

      DAPI

      440 nm

      YES

      diode laser

      CyanFP

      488nm

      YES

      diode laser

      GFP, Alexa488, CY2

      514 nm

      YES

      diode laser

      YellowFP

      555nm

      YES

      diode laser

      RedFPs, Alexa568

      639nm

      YES

      diode laser

      DRAQ5, CY5, Alexa648

      FILTERCUBES for Zeiss stand

      Fluorochromes

      Availability

      Excitation

      Dichroic

      Emission

      Filter No

      DAPI

      YES

      387 / 11-25

      409

      447 / 60-25

      DAPI-1160A

      GFP

      YES

      472 / 30-25

      495

      520 / 35-25

      GFP-3035

      CFP

      YES

      438 / 24-25

      458

      483 / 32-25

      CFP-2432

      Cy3

      YES

      531 / 40-25

      562

      593 / 40-25

      Cy3-4040A

      Cy5

      On Demand

      628 / 40-25

      660

      692 / 40-25

      Cy5-4040A

      YFP

      On Demand

      500 / 24-25

      520

      542 / 27-25

      YFP-2427A

      BANDPASS FILTERS (Emitters)

      Fluorochromes

      Availability

      Bandpass

      Emission

      Position No

      DAPI/FITC/Cy3/Cy5

      YES

      Multi-band

      440 / 521 / 607 / 700-25

      1

      DAPI

      On Demand

      Single-band

      445 / 45-25

      On Demand

      CFP

      YES

      Single-band

      482 / 35-25

      3

      GFP

      YES

      Single-band

      525 / 30-25

      4

      YFP

      YES

      Single-band

      542 / 27-25

      5

      RFP

      YES

      Single-band

      617 / 73-25

      6

      Cy5

      YES

      Long Pass

      665 - 1200

      2

      Cy5

      On Demand

      Single-band

      725 / 150-25

      On demand

       

      Location:

      Room 184 
      Imaging Core Facility (IMCF)
      Biozentrum
      Basel University

      Contact Specialist:

      Oliver Biehlmaier
      Biozentrum, Basel University
      Phone +41 61 207 20 73
      E-Mail oliver.biehlmaier@unibas.ch

      Usage Fee:

      Price:
      10 CHF / hour (Mo-Fr 09:00 - 17:00)
      5 CHF / hour (night and weekend)
      This is for your information only. MIAP
      is not responsible for correct usage fee
      information. For details and legally
      binding price information check the 
      undefinedUsage guidelines

      Documentation:

      no documents available